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Vitellogenin-like Proteins among Invertebrate Species Diversity: Potential of Proteomic Mass Spectrometry for Biomarker Development
journal contribution
posted on 2012-06-05, 00:00 authored by Guillaume Jubeaux, Fabien Audouard-Combe, Romain Simon, Renaud Tutundjian, Arnaud Salvador, Olivier Geffard, Arnaud ChaumotCost-effective methodologies along with cross-species
applicability
constitute key points for biomarker development in ecotoxicology.
With the advent of cheaper affordable genomic techniques and high
throughput sequencing, omics tools could facilitate the assessment
of effects of environmental contaminants for all taxa biodiversity.
We assessed the potential of absolute quantification of proteins using
mass spectrometry to develop vitellogenin(Vg)-like protein assays
for invertebrates. We used available sequences in public databases
to rapidly identify Vg-proteotypic peptides in seven species from
different main taxa of protostome invertebrates (mollusk bivalves,
crustacean amphipods, branchiopods, copepods and isopods, and insect
diptera). Functional validation was performed by comparing proteomic
signals from reproductive female tissue samples and negative controls
(male or juvenile tissues). In a second part, we demonstrate in gammarids,
daphnids, drosophilids, and gastropods that the assay validated in
Vg-sequenced species can be applied to Vg-unsequenced species thanks
to the evolutionary conservation of Vg-proteotypic peptide motifs.
Finally, we discuss the relevance of mass spectrometry for biomarker
development (specific measurement, rapid development, transferability
across species). Our study supplies an illustration of the promising
strategy to address the challenge of biodiversity in ecotoxicology,
which consists in employing omics tools from comparative and evolutionary
perspectives.