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Substrate Binding in Catechol Oxidase Activity:  Biomimetic Approach

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journal contribution
posted on 27.06.2002, 00:00 by Stéphane Torelli, Catherine Belle, Sylvain Hamman, Jean-Louis Pierre, Eric Saint-Aman
A series of dicopper(II) complexes have been investigated as model systems for the catechol oxidase active site enzyme, regarding the binding of catechol substrate in the first step of the catalytic cycle. The [Cu2(LR)(μ-OH)](ClO4)2 and [Cu2(LR)(H2O)2](ClO4)3 complexes are based on the LR ligands (2,6-bis[{bis(2-pyridylmethyl)amino}methyl]-4-R-substituted phenol) with −R = −OCH3, −CH3, or −F. Binding studies of diphenol substrates were investigated using UV−vis and EPR spectroscopy, electrochemistry, and 19F NMR (fluorinated derivatives). All the complexes are able to bind two ortho-diphenol substrates (tetrachlorocatechol and 3,5-di-tert-butylcatechol). Two successive fixation steps, respectively fast and slower, were evidenced for the μ-OH complexes (the bis(aqua) complexes are inactive in catalysis) by stopped-flow measurement and 19F NMR. From the μ-OH species, the 1:1 complex/substrate adduct is the catalytically active form. In relation with the substrate specificity observed in the enzyme, different substrate/inhibitor combinations were also examined. These studies enabled us to propose that ortho-diphenol binds monodentately one copper(II) center with the concomitant cleavage of the OH bridge. This hydroxo ligand appears to be a key factor to achieve the complete deprotonation of the catechol, leading to a bridging catecholate.

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