Specific Buffer Effects on the Intermolecular Interactions among Protein Molecules at Physiological pH

BSA and lysozyme molecular motion at pH 7.15 is buffer-specific. Adsorption of buffer ions on protein surfaces modulates the protein surface charge and thus protein–protein interactions. Interactions were estimated by means of the interaction parameter k_D obtained from plots of diffusion coefficients at different protein concentrations (D_app = D₀[1 + k_DC_protein]) via dynamic light scattering and nuclear magnetic resonance. The obtained results agree with recent findings confirming doubts regarding the validity of the Henderson–Hasselbalch equation, which has traditionally provided a basis for understanding pH buffers of primary importance in solution chemistry, electrochemistry, and biochemistry.