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Pea Protein/Gold Nanocluster/Indocyanine Green Ternary Hybrid for Near-Infrared Fluorescence/Computed Tomography Dual-Modal Imaging and Synergistic Photodynamic/Photothermal Therapy
journal contribution
posted on 2019-08-07, 12:37 authored by Mi Wu, Zhao Li, Jinrong Yao, Zhengzhong Shao, Xin ChenDevelopments in technology
such as theranostic nanoplatforms, which
combine complementary imaging modalities and synergistic therapies,
have brought new hope to the diagnosis and treatment of cancer, one
of the most deadly diseases. In this article, a nanoplatform (AuNCs/PPI-ICG
nanohybrid) with a theranostic effect was prepared using indocyanine
green (ICG), a cyanine dye with bright near-infrared fluorescence
(NIRF) and excellent photodynamic and photothermal performance, and
it was loaded onto gold nanoclusters/pea protein isolate hybrid nanoparticles
(AuNCs/PPI NPs). There was a linear relationship between ICG loading
ratio and the concentration, within a concentration range of 0–5
mg/mL, making it possible to precisely control the concentration of
loaded ICG. In addition, the AuNCs/PPI-ICG nanohybrid displayed outstanding
loading stability and enhanced the aqueous stability of ICG, owing
to the protectivity of AuNCs/PPI NPs. In vitro cell
experiments showed that this biocompatible nanohybrid could be taken
up by A549 cells and used as a fluorescent bioimaging probe. Moreover,
because of Au, AuNCs/PPI NPs could also act as a potential computed
tomographic (CT) contrast agent, and so the AuNCs/PPI-ICG nanohybrid
had the potential to function as a dual-modal imaging agent. Finally,
the as-prepared hybrid, with excellent photodynamic properties and
exceptional photothermal capabilities, showed a satisfactory therapeutic
effect in ablating A549 cancer cells. These results convincingly demonstrate
that the AuNCs/PPI-ICG nanohybrid is a promising theranostic nanoplatform
for NIRF/CT dual-modal imaging and for synergistic photodynamic therapy/photothermal
therapy (PDT/PTT).
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Keywords
biocompatible nanohybriddual-modal imaging agentphotothermal capabilitiestheranostic nanoplatformconcentration rangetherapycell experimentsNIRF549 cancer cellsimaging modalitiesAuNCphotothermal performancenear-infrared fluorescencetheranostic effectNPPDTCTcyanine dye549 cellscontrast agentloading stabilityICG loading ratiotheranostic nanoplatformsbioimaging probephotodynamic properties