am4045713_si_001.pdf (11.22 MB)
Mimicking Red Blood Cell Lipid Membrane To Enhance the Hemocompatibility of Large-Pore Mesoporous Silica
journal contribution
posted on 2014-02-12, 00:00 authored by Robert
A. Roggers, Madhura Joglekar, Justin S. Valenstein, Brian G. TrewynMesoporous silica nanoparticles (MSNs)
have been repeatedly demonstrated
as potential drug-delivery devices. The study of biocompatibility
and interaction of these materials with the various cell types is
of great interest with regard to the development of viable pharmaceutical
products. By mimicking the cholesterol, phosphatidylcholine, and phosphatidylethanolamine
composition of the outer leaflet of a human red blood cell (RBC),
lipid-bilayer-coated mesoporous silica particles show considerably
improved hemocompatibility over phosphatidylcholine-coated and uncoated
large-pore MSN (l-MSN). These inorganic/organic composite
nanomaterials are shown to be capable of interfacing with RBCs without
damaging the cells even at relatively high concentrations, as observed
through electron microscopy, UV–vis spectroscopy, and flow
cytometry analyses. Interestingly, the absence of cholesterol in the
outer bilayer composition is shown to produce toxic effects without
resulting in hemolysis. By maintaining the ζ potential of lipid-bilayer-functionalized
MSNs similar to that of the hemolytic l-MSNs, we
demonstrate that the bilayer composition, and not the surface charge,
plays a significant role in determining the hemocompatibility of MSN-based
materials.