Microfluidic Electrochemical Sensor for Cerebrospinal Fluid and Blood Dopamine Detection in a Mouse Model of Parkinson’s Disease
journal contributionposted on 2020-08-26, 20:11 authored by Mehmet Senel, Esma Dervisevic, Sammy Alhassen, Muamer Dervisevic, Amal Alachkar, Victor J. Cadarso, Nicolas H. Voelcker
Parkinson’s disease (PD) is a progressive neurodegenerative disorder involving dopaminergic neurons from the substantia nigra. The loss of dopaminergic neurons results in decreased dopamine (DA) release in the striatum and thus impaired motor functions. DA is one of the key neurotransmitters monitored for the diagnosis and during the progression and treatment of PD. Therefore, sensitive and selective DA detection methods are of high clinical relevance. In this study, a new microfluidic device utilized for electrochemical DA detection is reported. The microfluidic sensing device operates in the range of 0.1–1000 nM DA requiring only ∼2.4 μL sample volume, which corresponds to detectable 240 amol of DA. Using this sensor, we were able to monitor the changes in DA levels in cerebrospinal fluid and plasma of a mouse model of PD and following the treatment of drug l-3,4-dihydroxyphenylalanine.
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DA levelsmicrofluidic devicedopaminergic neuronsDA detection methodsneurodegenerative disordercerebrospinal fluidCerebrospinal Fluidmotor functionsMicrofluidic Electrochemical SensorPDelectrochemical DA detectiondopaminergic neurons resultsmouse modelsubstantia nigraBlood Dopamine Detection240 amolMouse Model