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Ion-Pair Amphiphile: A Neoteric Substitute That Modulates the Physicochemical Properties of Biomimetic Membranes
journal contribution
posted on 2015-03-19, 00:00 authored by Pritam Guha, Biplab Roy, Gourab Karmakar, Prasant Nahak, Suraj Koirala, Manish Sapkota, Takeshi Misono, Kanjiro Torigoe, Amiya Kumar PandaIon-pair amphiphiles (IPAs) are neoteric
pseudo-double-tailed compounds
with potential as a novel substitute of phospholipid. IPA, synthesized
by stoichiometric/equimolar mixing of aqueous solution of hexadecyltrimethylammonium
bromide (HTMAB) and sodium dodecyl sulfate (SDS), was used as a potential
substituent of naturally occurring phospholipid, soylecithin (SLC).
Vesicles were prepared using SLC and IPA in different ratios along
with cholesterol. The impact of IPA on SLC was examined by way of
surface pressure (π)–area (A) measurements.
Associated thermodynamic parameters were evaluated; interfacial miscibility
between the components was found to depend on SLC/IPA ratio. Solution
behavior of the bilayers, in the form of vesicles, was investigated
by monitoring the hydrodynamic diameter, zeta potential, and polydispersity
index over a period of 100 days. Size and morphology of the vesicles
were also investigated by electron microscopic studies. Systems comprising
20 and 40 mol % IPA exhibited anomalous behavior. Thermal behavior
of the vesicles, as scrutinized by differential scanning calorimetry,
was correlated with the hydrocarbon chain as well as the headgroup
packing. Entrapment efficiency (EE) of the vesicles toward the cationic
dye methylene blue (MB) was also evaluated. Vesicles were smart enough
to entrap the dye, and the efficiency was found to vary with IPA concentration.
EE was found to be well above 80% for some stable dispersions. Such
formulations thus could be considered to have potential as novel drug
delivery systems.
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Thermal behaviorsodium dodecyl sulfatehexadecyltrimethylammonium bromide100 daysEEHTMABscanning calorimetryhydrodynamic diameterhydrocarbon chainvesicleSuch formulationsnovel drug delivery systemscationic dye methyleneMBnovel substitutePhysicochemical Propertiespolydispersity indexsolution behaviorIPA concentrationSLCSDSEntrapment efficiency
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