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Enzymatic Preparation of Plasmonic-Fluorescent Quantum Dot-Gold Hybrid Nanoprobes for Sensitive Detection of Glucose and Alkaline Phosphatase and Dual-Modality Cell Imaging
journal contribution
posted on 2019-10-18, 19:14 authored by Jie Zhang, Guohua Qi, Chen Xu, Yongdong JinHerein, we develop
a route to prepare bifunctional plasmonic-fluorescent
quantum dot-gold (QD-Au) hybrid nanoprobes by use of enzymatic reactions.
Two bioenzymes, glucose oxidase (GOx) and alkaline phosphatase (ALP)
were chosen for the enzymatic preparation of core–satellite
or core–shell type CdSe/ZnS@Au hybrid nanostructures. The enzymatic
products, H2O2 and l-ascorbic acid,
of the two enzymes were exploited as mild reducing agents for controlled
Au deposition on QD surfaces. The polymer multilayers by layer-by-layer
assembly were used to adjust the separation between QD core and plasmonic
Au, which can effectively reduce the quenching effect of the Au on
QDs. The as-prepared QD@Au hybrid nanostructures are excellent dual-modality
imaging nanoprobes, and can be used for fluorescence and dark-field
scattering dual-imaging of MCF-7 cells. More importantly, the two
enzymatic reaction systems can be explored for sensitive and selective
detection of glucose and alkaline phosphatase, respectively, by monitoring
the fluorescence spectra change of QD@Au hybrid nanoparticles, which
is very useful for the glucose- and ALP-related disease diagnosis.
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Keywords
bifunctional plasmonic-fluorescent quantum dot-goldlayer-by-layer assemblyl-ascorbic acidquenching effectALP-related disease diagnosisreaction systemsQD coreQD surfacesphosphataseEnzymatic PreparationMCF -7 cellsSensitive Detectionglucose oxidasedual-modality imaging nanoprobespolymer multilayersfluorescence spectra changeDual-Modality Cell Imaging HereinH 2 O 2Alkaline PhosphatasenanostructurePlasmonic-Fluorescent Quantum Dot-Gold Hybrid Nanoprobes
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