Direct Plant Tissue Analysis and Imprint Imaging by Desorption Electrospray Ionization Mass Spectrometry
journal contributionposted on 16.12.2015, 19:26 by Thomas Müller, Sheran Oradu, Demian R. Ifa, R. Graham Cooks, Bernhard Kräutler
The ambient mass spectrometry technique, desorption electrospray ionization mass spectrometry (DESI-MS), is applied for the rapid identification and spatially resolved relative quantification of chlorophyll degradation products in complex senescent plant tissue matrixes. Polyfunctionalized nonfluorescent chlorophyll catabolites (NCCs), the “final” products of the chlorophyll degradation pathway, are detected directly from leaf tissues within seconds and structurally characterized by tandem mass spectrometry (MS/MS) and reactive-DESI experiments performed in situ. The sensitivity of DESI-MS analysis of these compounds from degreening leaves is enhanced by the introduction of an imprinting technique. Porous polytetrafluoroethylene (PTFE) is used as a substrate for imprinting the leaves, resulting in increased signal intensities compared with those obtained from direct leaf tissue analysis. This imprinting technique is used further to perform two-dimensional (2D) imaging mass spectrometry by DESI, producing well-resolved images of the spatial distribution of NCCs in senescent leaf tissues.
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tandem mass spectrometryPTFEDesorption Electrospray Ionization Mass SpectrometryThe ambient mass spectrometry techniqueMSleaf tissue analysisPolyfunctionalized nonfluorescent chlorophyll catabolitesdesorption electrospray ionization mass spectrometrysenescent plant tissue matrixeschlorophyll degradation productsimprinting techniquesenescent leaf tissuesimaging mass spectrometryDESIchlorophyll degradation pathwayNCCDirect Plant Tissue Analysis