posted on 2020-08-20, 15:41authored byKe Li, Yi Qu, Yulong An, Eric Breinlinger, Matthew P. Webster, Huanan Wen, Duanchen Ding, Meng Zhao, Xiaodong Shi, Jiangong Wang, Wenji Su, Weiren Cui, Alexander L. Satz, Hongfang Yang, Letian Kuai, Andrew Little, Xuanjia Peng
We
report a DNA-compatible protocol for synthesizing amides from
DNA-bound aldehydes and non-nucleophilic arylamines including aza-substituted
anilines, 2-aminobenzimidazoles, and 3-aminopyrazoles. The reactions
were carried out at room temperature and provided reasonable conversions
and wide functional group compatibility. The reactions were also successful
when employing aryl and aliphatic aldehydes. In addition, qPCR and
NGS data suggested no negative impact on DNA integrity after the copper-mediated
oxidative amidation reaction.