American Chemical Society
mp6b00716_si_001.pdf (472.71 kB)

Characterization of Lipid-Based Hexosomes as Versatile Vaccine Carriers

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journal contribution
posted on 2016-09-08, 00:00 authored by Letícia Rodrigues, Konstantinos Kyriakos, Fabian Schneider, Hendrik Dietz, Gerhard Winter, Christine M. Papadakis, Madlen Hubert
Subunit vaccines typically show insufficient immunogenicity. To address this issue, we developed a novel self-adjuvanting particulate carrier system based upon the lipids phytantriol (Phy) and mannide monooleate (MaMo). Phy is a lipid known to form nonlamellar phases in fully hydrated systems, whereas MaMo has been found to promote immune responses in emulsion form. A bulk phase composition of Phy/MaMo (14 wt %) showed hexagonal (HII) phase behavior over a practical temperature range (including room and body temperature), and was therefore used for particle development. Hexosomes stabilized with different concentrations of either poloxamer 407, Myrj 59, or Pluronic F108 were successfully prepared. To demonstrate the versatile nature of these systems, the particles were further modified with either positively or negatively charged lipids and loaded with model antigens, while maintaining the HII structure. These hexosomes are structurally robust and amenable to customization, rendering them suitable as antigen delivery carriers.