Catalytic Activity, Stability, and Loading Trends of Alcohol Dehydrogenase Enzyme Encapsulated in a Metal–Organic Framework
journal contributionposted on 24.09.2021, 19:37 by Josh Phipps, Hao Chen, Connor Donovan, Dylan Dominguez, Sydney Morgan, Barrett Weidman, Chenguang Fan, Hudson Beyzavi
Recently, it has been shown that enzyme encapsulation inside metal–organic frameworks (MOFs) can increase enzyme activity and serve as protection from adverse environmental conditions. Little is understood about how the enzymes move into and are held inside the MOFs although it is believed that intermolecular forces between the MOF and the enzyme cause it to be held in place. If this process can be better understood, it can have dramatic implications on the cost-effectiveness and implementation of enzyme–MOF complexes. This is of specific importance in the medical sector for protein therapy and the industrial sector where enzyme use is expected to increase. Herein, we synthesized alcohol dehydrogenase (ADH) and PCN-333 to study encapsulation, stability, and enzyme activity to expand the knowledge of our field and offer a potential improvement to a synthetic route for biofuel synthesis. From this, we found a correlation between the concentration of a buffer and the loading of an enzyme, with surprising loading trends. We conclude that the buffer solution decreases interactions between the enzyme and MOF, supporting conventional theory and allowing it to penetrate deeper into the structure causing higher enzyme loading while allowing for excellent stability over time at various pH values and temperatures and after multiple reactions. We also observe new trends such as a rebounding effect in loading and “out-of-bounds” reactions.
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various ph valuessynthesized alcohol dehydrogenasesupporting conventional theoryadverse environmental conditionsbounds ” reactionssurprising loading trendsincrease enzyme activitymultiple reactionsloading trendsenzyme activitycatalytic activitysynthetic routestudy encapsulationspecific importancerebounding effectprotein therapypotential improvementpenetrate deepermedical sectorintermolecular forcesindustrial sectorenzymes moveenzyme useenzyme causedramatic implicationsbiofuel synthesis