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Blue Flickers of Hope: Secondary Structure, Dynamics, and Putative Dimerization Interface of the Blue-Light Receptor YtvA from Bacillus subtilis
journal contribution
posted on 2011-09-27, 00:00 authored by Marcel Jurk, Matthias Dorn, Peter SchmiederBacillus subtilis is capable of responding
to
various kinds of extracellular, potentially harmful stimuli via a
stress response pathway, which involves a signal transduction and
integration hub, the stressosome, and finally leads to activation
of σB. One of the different signals initiating the
underlying phosphorylation cascade is blue light. While it is known
that the bacterial photoreceptor YtvA is responsible for blue light
detection, the intramolecular activation mechanism and the structure
of this multidomain protein are unknown. Using solution NMR spectroscopy,
we have obtained a near complete backbone assignment of the full-length
protein. More importantly, we report relaxation data and data on the
solvent accessibility of full-length YtvA in the dark state which
are interpreted with respect to secondary structure, the mobility,
and the quaternary structure of the protein. Finally, we show that
YtvA adopts an elongated domain orientation with LOV–LOV and
STAS–STAS interactions on either side.
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Keywords
signal transductionσ Bdomain orientationLOVintegration hubphosphorylation cascadelight detectionstress response pathwayBlue FlickersSTASquaternary structurephotoreceptor YtvAintramolecular activation mechanismPutative Dimerization InterfaceBacillus subtilisBacillus subtilisbackbone assignmentmultidomain proteinsolution NMR spectroscopySecondary Structurereport relaxation data
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