American Chemical Society
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An Experimentally Derived Database of Candidate Ras-Interacting Proteins

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journal contribution
posted on 2020-04-02, 16:19 authored by Lawrence E. Goldfinger, Celeste Ptak, Erin D. Jeffery, Jeffrey Shabanowitz, Jaewon Han, Jacob R. Haling, Nicholas E. Sherman, Jay W. Fox, Donald F. Hunt, Mark H. Ginsberg
We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases:  H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells. Keywords: proteomics • mass spectrometry • GTPases • Ras