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Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix
journal contribution
posted on 2013-02-19, 00:00 authored by Zhihua Wang, Xu Teng, Chao LuIn this work, a universal chemiluminescence (CL) flow-through
device
suitable for various CL resonance energy transfer (CRET) systems has
been successfully fabricated. Highly efficient CRET in solid-state
photoactive organic molecules can be achieved by assembling them on
the surface of layered double hydroxides (LDHs). We attribute these
observations to the suppression of the intermolecular π–π
stacking interactions among aromatic rings and the improvement of
molecular orientation and planarity in the LDH matrix, enabling a
remarkable increase in fluorescence lifetime and quantum yield of
organic molecules. Under optimal conditions, using peroxynitrous acid–fluorescein
dianion (FLUD) as a model CRET system, trace FLUD (10 μM) was
assembled on the surface of LDHs. Peroxynitrous acid/nitrite could
be assayed in the range of 1.0–500 μM, and the detection
limit for peroxynitrous acid/nitrite (S/N = 3) was 0.6 μM. This
CL flow-through device exhibited operational stability, high reproducibility,
and long lifetime. While LDHs were immobilized in a flow-through device
in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite
was 100 μM. On the other hand, FLUD at the same concentration
can not enhance the CL intensity of peroxynitrous acid system. This
fabricated CL flow-through column has been successfully applied to
determine nitrite in sausage samples with recoveries of 98–102%.
These satisfactory results demonstrated that our studies pave a novel
way toward flow-through column-based CRET using solid-state organic
molecules as acceptors for signal amplification.