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Size-Controlled Preparation of Microsized Perfluorocarbon Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane Emulsification Technique
journal contribution
posted on 2019-02-21, 00:00 authored by Xiaoting Fu, Seiichi Ohta, Masamichi Kamihira, Yasuyuki Sakai, Taichi ItoWe have developed
microsized perfluorocarbon (PFC) emulsions with
different sizes as artificial oxygen carriers (OCs) via Shirasu porous
glass membrane emulsification. Monodispersed PFC emulsions with narrow
size distribution were obtained. By changing the membrane pore size,
we were able to precisely control the size of emulsions and fabricate
emulsions similar in size to human red blood cells. Behaviors of Pluronics
with different physiochemical properties (F127, F68, P85, and P103)
as surfactants were also investigated, which evidenced that the type
and concentration of Pluronics have a major impact on the size of
emulsions and the response to different thermal conditions. The F127-stabilized
microsized PFC emulsions were stable even during autoclave sterilization.
The emulsions were loaded with Ru(ddp)an oxygen-sensitive
probeon their surfaces to indicate oxygen concentration. Finally,
incubations with HeLa cells that show fluorescence in response to
hypoxia cultured in 2D and 3D suggested promising potential of our
emulsions for OCs.
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oxygen concentrationOCautoclave sterilizationOxygen Carriersshow fluorescenceSize-Controlled Preparationblood cellssize distributionHeLa cellsoxygen carriersglass membrane emulsificationMicrosized Perfluorocarbon Emulsionsmicrosized perfluorocarbon2 DMonodispersed PFC emulsionsShirasu Porous Glass Membrane Emulsification TechniqueF 127-stabilized microsized PFC emulsions3 Dmembrane pore size
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