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Nucleic Acid-Functionalized Metal–Organic Framework-Based Homogeneous Electrochemical Biosensor for Simultaneous Detection of Multiple Tumor Biomarkers
journal contribution
posted on 2019-02-13, 00:00 authored by Jiafu Chang, Xin Wang, Jiao Wang, Haiyin Li, Feng LiSimultaneous detection
of multiple tumor biomarkers is in great
demand for early and accurate cancer diagnosis. A homogeneous electrochemical
biosensor has been proven to possess high sensitivity, but achieving
simultaneous detection of multiple tumor biomarkers is still a challenge.
Herein, we develop a novel homogeneous electrochemical biosensor for
simultaneous detection of multiple tumor biomarkers based on the functionalized
metal–organic frameworks (MOFs). The functionalized MOFs were
prepared by using porous UIO-66-NH2 as nanocontainer to
load electroactive dyes and dsDNA as a gatekeeper to cap MOFs. In
this context, two functionalized MOFs (MB@UIO and TMB@UIO) were fabricated
and applied to simultaneous detection of let-7a and miRNA-21, used
as the proof-of-concept analytes. The recognition and hybridization
of PX with target miRNAs impel the generation of RNA–DNA
complexes, which separated from MOFs and allowed the electroactive
dyes to be released. In comparison with the case when target miRNAs
are absent, two stronger signals are recorded, and dependent on target
miRNA concentrations. Thus, simultaneous detection of let-7a and minRNA-21
is achieved, with detection limits down to 3.6 and 8.2 fM, respectively,
comparable or lower than those of reported strategies that concentrated
on single miRNA detection. Moreover, the proposed biosensor has also
been successfully applied for simultaneous detection of target miRNAs
spiked in serum samples. Therefore, the proposed strategy was expected
to provide more information for early and accurate cancer diagnosis
and was an useful application in disease diagnosis and clinical biomedicine.
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- Biophysics
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