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Multi-laboratory Validation of a New Marine Biodegradation Screening Test for Chemical Persistence Assessment
journal contribution
posted on 2020-03-23, 11:37 authored by Amelie Ott, Timothy J. Martin, Kishor Acharya, Delina Y. Lyon, Nik Robinson, Bob Rowles, Jason R. Snape, Ian Still, Graham F. Whale, Vurtice C. Albright, Petra Bäverbäck, Nicola Best, Ruth Commander, Curtis Eickhoff, Sarah Finn, Björn Hidding, Heiko Maischak, Katherine A. Sowders, Masanori Taruki, Helen E. Walton, Aina Charlotte Wennberg, Russell J. DavenportCurrent biodegradation screening
tests are not specifically designed
for persistence assessment of chemicals, often show high inter- and
intra-test variability, and often give false negative biodegradation
results. Based on previous studies and recommendations, an international
ring test involving 13 laboratories validated a new test method for
marine biodegradation with a focus on improving the reliability of
screening to determine the environmental degradation potential of
chemicals. The new method incorporated increased bacterial cell concentrations
to better represent the microbial diversity; a chemical is likely
to be exposed in the sampled environments and ran beyond 60 days,
which is the half-life threshold for chemical persistence in the marine
environment. The new test provided a more reliable and less variable
characterization of the biodegradation behavior of five reference
chemicals (sodium benzoate, triethanolamine, 4-nitrophenol, anionic
polyacrylamide, and pentachlorophenol), with respect to REACH and
OSPAR persistence thresholds, than the current OECD 306 test. The
proposed new method provides a cost-effective screening test for non-persistence
that could streamline chemical regulation and reduce the cost and
animal welfare implications of further higher tier testing.
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marine environmentmarine biodegradationbiodegradation behaviorOECD 306 testOSPAR persistence thresholdstier testingchemical regulationscreening testMulti-laboratory Validationcell concentrationsbiodegradation results4- nitrophenolreference chemicalshalf-life thresholdbiodegradation screening testsChemical Persistence Assessment60 daystest methodNew Marine Biodegradation Screening Testsodium benzoateREACHintra-test variabilitypersistence assessmentring test13 laboratoriesanimal welfare implicationschemical persistence
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