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Metal Recognition of Septapeptides via Polypod Molecular Architecture
journal contribution
posted on 2005-03-09, 00:00 authored by Ersin Emre Oren, Candan Tamerler, Mehmet SarikayaThe understanding of the nature of recognition of inorganic materials by proteins is one of the core elements of and has profound implications
in biological materials science and engineering. Using combinatorial display methods, a considerable number of short polypeptides have
recently been selected with affinity to engineering materials. During these selections, more than several polypeptides are identified with
binding specificity to a chosen inorganic material. Understanding the nature of surface recognition of materials by polypeptides is essential
for rational design and biomimetic engineering of these inorganic-binding polypeptides for use as linkers, catalyzers, and growth modifiers
in nanotechnology and nanobiotechnology. Although there may not be direct homology among the amino acids constituting the polypeptides,
their function may come from conserved molecular architecture. Here we study crystallographic surface recognition of platinum metal-binding
septapeptides by conformational analysis. We find that the septapeptides conform into certain molecular architectures containing multiple
protrusions (polypods) that spatially match with the crystallographic metal surfaces. While the physical recognition may originate from how
well the molecular polypods spatially match a given crystallographic surface, the degree of binding may be due to the reactive groups that
form the polypods, e.g., charged or polar groups (e.g., hydroxyl and amine). These results are highly consistent with the experimental binding
characteristics of the Pt binders with various degrees of affinities.
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g ., hydroxylg ., chargeddirect homology amongconserved molecular architectureamino acids constitutinggiven crystallographic surfacecrystallographic metal surfacesexperimental binding characteristicsfunction may comechosen inorganic materialbiological materials scienceplatinum metalbinding specificitybinding septapeptidesbinding maysurface recognitionvarious degreesspatially matchseptapeptides conformreactive groupsrational designpt bindersprofound implicationspolar groupsmetal recognitioninorganic materialshighly consistentgrowth modifierscore elementsconsiderable numberconformational analysisamine ).
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