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Metabolic Traits of Candidatus Accumulibacter clade IIF Strain SCELSE‑1 Using Amino Acids As Carbon Sources for Enhanced Biological Phosphorus Removal
journal contribution
posted on 2019-12-16, 05:32 authored by Guanglei Qiu, Xianghui Liu, Nay Min Min Thaw Saw, Yingyu Law, Rogelio Zuniga-Montanez, Sara Swa Thi, Thi Quynh Ngoc Nguyen, Per H. Nielsen, Rohan B.H. Williams, Stefan WuertzDespite recent evidence from full-scale plants suggesting
that Candidatus Accumulibacter may be capable of
using amino
acids, this metabolic trait has never been confirmed in a bioreactor
experiment. Here we show that an enriched culture of Ca. Accumulibacter clade IIF strain SCELSE-1 could metabolize 11 of
20 α-amino acids, with aspartate, glutamate, asparagine, and
glutamine resulting in the highest phosphorus removal. The anaerobic
uptake of aspartate and glutamate was achieved through a glutamate/aspartate-proton
symporter fully powered by the proton motive force (PMF). Under anaerobic
conditions aspartate was deaminized and routed into core carbon metabolic
pathways to form polyhydroxyalkanoates (PHA). The lack of genes encoding
NADH dependent isocitrate dehydrogenase in the Ca. Accumulibacter genome resulted in a kinetic barrier for glutamate
to be channelled to the TCA cycle. Glutamate was stored as glutamate
polymer. When amino acids (aspartate or glutamate) and acetate were
supplied together, Ca. Accumulibacter took up both
carbon sources simultaneously, with the uptake rate of each carbon
source largely preserved. Overall energy savings (up to 17%) were
achieved under mixed carbon scenarios, due to the ability of Ca. Accumulibacter to rearrange its anaerobic carbon metabolism
based on the reducing power, PMF and ATP balance.
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Accumulibacter clade IIF strain SCELSEuptake rateglutamate polymerPHAMetabolic Traitsisocitrate dehydrogenasegenes encoding NADHcarbon metabolismacid20 α-carbon scenariosconditions aspartateform polyhydroxyalkanoatesbioreactor experimentcarbon sourcephosphorus removalcarbon sourcesAmino AcidsTCA cyclePMFcore carbonPhosphorus RemovalCandidatus AccumulibacterATP balanceproton motive forceenergy savingsCarbon SourcesAccumulibacter genome
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