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Magnetic Oleosome as a Functional Lipophilic Drug Carrier for Cancer Therapy
journal contribution
posted on 2018-02-28, 00:00 authored by Hyeon-Yeol Cho, Taek Lee, Jinho Yoon, Zhenlin Han, Hudifah Rabie, Ki-Bum Lee, Wei Wen Su, Jeong-Woo ChoiIn
the present study, we fabricated magnetic oleosomes functionalized
with recombinant proteins as a new carrier for oil-based lipophilic
drugs for cancer treatment. The bioengineered oleosome is composed
of neutral lipids surrounded by a phospholipid monolayer with embedded
oleosin fusion proteins. The oleosin was genetically fused to a nanobody
of a green fluorescent protein (GFP). A recombinant protein consisting
of immunoglobulin-binding protein LG fused to GFP was used to couple
the oleosome to an antibody for targeted delivery to breast cancer
cells. The lipid core of the oleosome was loaded with magnetic nanoparticles
and carmustine as the lipophilic drug. The magnetic oleosome was characterized
using transmission electron microscopy and dynamic light scattering.
Moreover, the specific delivery of oleosome into the target cancer
cell was investigated via confocal microscopy. To examine the cell
viability of the delivered oleosome, a conventional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide assay was carried out. Furthermore, an animal study was conducted
to confirm the effect resulting from the delivery of the anticancer
drug-loaded oleosomes. Taken together, the fabricated lipophilic drug-loaded
magnetic oleosome can be a powerful tool for oil-based drug delivery
agent for cancer therapy.
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confocal microscopylipophilic drugslipophilic drugFunctional Lipophilic Drug Carrierbioengineered oleosomecancer therapyimmunoglobulin-binding protein LGlipid corecell viabilityphospholipid monolayerCancer Therapydrug delivery agentoleosin fusion proteinsoleosomes functionalizedcancer treatmentGFPanticancer drug-loaded oleosomesMagnetic Oleosometransmission electron microscopyanimal studylipophilic drug-loadedtarget cancer cellbreast cancer cells
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