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Highly Efficient and Enzyme-Recoverable Method for Enzymatic Concentrating Omega‑3 Fatty Acids Generated by Hydrolysis of Fish Oil in a Substrate-Constituted Three-Liquid-Phase System
journal contribution
posted on 2019-02-11, 00:00 authored by Zhigang Li, Hua Chen, Jinfen Su, Weifei Wang, Huayong Chen, Bo Yang, Yonghua WangA novel
three-liquid-phase system which contained fish oil as the
nonpolar phase was developed for the lipase-based hydrolysis of fish
oil and subsequent enrichment of the omega-3 polyunsaturated fatty
acids (n-3 PUFA) in the glyceride fraction of the fish oil. In comparison
with the traditional oil/water system, the enrichment factor of n-3
PUFA in this system was increased by 363.4% as a result of a higher
dispersity, higher selectivity of the lipase for the other fatty acids
except for n-3PUFA, and relief of product
inhibition. The content of n-3 PUFA in the glyceride fraction could
be concentrated to 67.97% by repeated hydrolysis after removing the
free fatty acids. Furthermore, the lipase could be reused for at least
eight rounds. This method would be an ideal approach for enriching
n-3 PUFA because it is cost-effective, low in toxicity, and easily
scaled up.