jf9b04620_si_001.pdf (792 kB)
Diketopiperazines Synthesis Gene in Shewanella baltica and Roles of Diketopiperazines and Resveratrol in Quorum Sensing
journal contribution
posted on 2019-10-18, 19:18 authored by Junli Zhu, Yuwei Zhang, Jingmin Deng, Hanyun Jiang, Liumin Zhuang, Wei Ye, Jiayu Ma, Jingyang Jiang, Lifang FengThe
synthesis pathways of quorum sensing (QS) signal molecules
and the mechanism of action of quorum sensing inhibitors (QSIs) have
gained considerable attention as research topics in the field of food
preservation. Here, Shewanella baltica was detected
as the specific spoilage organism in large yellow croaker during 4
°C storage, and it produced the QS signal molecules autoinducer-2
(AI-2) and diketopiperazines (DKPs). Then, a cyclodipeptide synthase
(CDPS) homologous gene, sb1370, was screened, and
knockout and rescue results revealed that this gene was involved in
DKP synthesis but not in AI-2 synthesis, and it also played an important
role in QS. Furthermore, fish fillets and mutant strains were treated
with resveratrol, and the results suggested that resveratrol was an
ideal QSI for inhibition of DKPs production via the sb1370 gene and reduced QS in S. baltica, thus delaying
the process of fish spoilage during chilling storage.