jm9b02042_si_001.pdf (2.7 MB)
Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe
journal contribution
posted on 2020-03-16, 18:04 authored by Sonia Kołt, Tomasz Janiszewski, Dion Kaiserman, Sylwia Modrzycka, Scott J. Snipas, Guy Salvesen, Marcin Dra̧g, Phillip I. Bird, Paulina KasperkiewiczCytotoxic
T-lymphocytes (CTLs) and natural killer cells (NKs) kill
compromised cells to defend against tumor and viral infections. Both
effector cell types use multiple strategies to induce target cell
death including Fas/CD95 activation and the release of perforin and
a group of lymphocyte granule serine proteases called granzymes. Granzymes
have relatively broad and overlapping substrate specificities and
may hydrolyze a wide range of peptidic epitopes; it is therefore challenging
to identify their natural and synthetic substrates and to distinguish
their localization and functions. Here, we present a specific and
potent substrate, an inhibitor, and an activity-based probe of Granzyme
A (GrA) that can be used to follow functional GrA in cells.