ab9b00926_si_001.pdf (299.61 kB)
Coaxial Extrusion of Tubular Tissue Constructs Using a Gelatin/GelMA Blend Bioink
journal contribution
posted on 2019-09-09, 20:48 authored by Ying Wang, Ranjith Kumar Kankala, Kai Zhu, Shi-Bin Wang, Yu Shrike Zhang, Ai-Zheng ChenGelatin
methacryloyl (GelMA) hydrogels have been commonly used
in fabricating cannular tissue constructs due to their excellent cytocompatibility,
as well as amenity to migration and proliferation of encapsulated
cells. Here, we present a simple yet efficient approach for fabricating
hollow structures with gelatin-based hydrogels using a modified microfluidic-based
biofabrication technology. Hollow microfibers were generated using
a customized coaxial nozzle by soft templating against a polyvinyl
alcohol solution core. Reversible thermo-cross-linking and irreversible
photo-cross-linking were achieved successively through gelatin (Gel)
mixed into the GelMA solution and by irradiating with UV light, resulting
in stable and continuous generation of hollow structures. Furthermore,
in vitro evaluations confirmed good proliferation of multiple cell
types in the GelMA/Gel hollow microfibers. Together, we believe that
this approach holds great potential in engineering cannular constructs
for applications in regenerative medicine and tissue modeling.
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cannular tissueregenerative medicineencapsulated cellsHollow microfibersReversible thermo-cross-linkingpolyvinyl alcohol solution corecell typesgelatin-based hydrogelsapproachproliferationCoaxial Extrusionmicrofluidic-based biofabrication technologyUV lightTubular Tissue Constructstissue modelingengineering cannularBioink Gelatin methacryloylGelMA solution
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