sc9b06850_si_001.pdf (1.14 MB)
Arsenic Toxicity: Carbonate’s Counteraction Revealed
journal contribution
posted on 2020-03-24, 01:13 authored by Swathy
Jakka Ravindran, Shantha Kumar Jenifer, Jayashree Balasubramanyam, Sourav Kanti Jana, Subramanian Krishnakumar, Sailaja Elchuri, Ligy Philip, Thalappil PradeepWell-known
purification technologies built for arsenic (As) removal
from drinking water are not sustainable, either being unaffordable
or inefficient in the elimination of traces of As. In our experiments,
we observed that carbonate ion can counteract the effects of As exposure
as it efficiently prevented As-induced cytotoxicity on epithelial
cell lines of the small intestine (IEC-6). The cotreatment of IEC-6
cells with 40 ppm of carbonates and As (≥3 ppm) showed substantial
remissions in the As-induced cytotoxicity and increased the viability
from 50% to 75%. The production of intracellular reactive oxygen species
(ROS) and cellular acidification were also reduced in this process
(pH increase from 5 to 6.5). Thus, the present study suggests that
the cytoprotective effect of carbonate can involve multiple pathways,
such as reduction of extracellular/intracellular acidosis, H2O2 decomposition, balancing mitochondrial potential, and
immobilization of As. We show that As-contaminated drinking water
enriched with carbonates up to 40 ppm has a reduced toxic effect on
cells in comparison to that of an As-alone sample. Therefore, carbonates
can act as an adjunct in addition to the prevailing approaches to
tackle mass poisoning by As. We believe that this study is initial
evidence for developing an alternative method to tackle the prevailing
mass environmental poisoning by As, using locally available, affordable,
safe, and sustainable solutions.