Annotated spectra

Annotated spectra of F_02_23_E1_ETD.msf

Workflow created with Discoverer version: 1.3.0.339 (DBVersion:78)

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Search name: F_02_23_E1
Search description: -
Search date: 09/13/2013 10:12:41

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The pipeline tree:
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    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Non-Fragment Filter
                |-(3) Mascot
                    |-(4) Percolator

================================================================================

Search name: F_02_23_E1
Search description: -
Search date: 09/13/2013 10:12:41

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Non-Fragment Filter
                |-(3) Mascot
                    |-(4) Percolator

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Processing node 0: Spectrum Files
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Input Data:
-----------------------------
File Name(s): F:\Cytosol\M15F15 022012\Raw files\F_02_23_E1.raw

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Processing node 1: Spectrum Selector
------------------------------------------------------------------------------

1. General Settings:
-----------------------------
Precursor Selection: Use MS1 Precursor
Use New Precursor Reevaluation: True

2. Spectrum Properties Filter:
-----------------------------
Lower RT Limit: 0
Upper RT Limit: 0
First Scan: 0
Last Scan: 0
Lowest Charge State: 0
Highest Charge State: 0
Min. Precursor Mass: 400 Da
Max. Precursor Mass: 10000 Da
Total Intensity Threshold: 0
Minimum Peak Count: 1

3. Scan Event Filters:
-----------------------------
MS Order: Is MS2
Activation Type: Is ETD
Scan Type: Is Full

4. Peak Filters:
-----------------------------
S/N Threshold (FT-only): 3

5. Replacements for Unrecognized Properties:
-----------------------------
Unrecognized Charge Replacements: Automatic
Unrecognized Mass Analyzer Replacements: ITMS
Unrecognized MS Order Replacements: MS2
Unrecognized Activation Type Replacements: ETD
Unrecognized Polarity Replacements: +

6. Just for Testing:
-----------------------------
Precursor Clipping Range Before: 2.5 Da
Precursor Clipping Range After: 5.5 Da

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Processing node 2: Non-Fragment Filter
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1. Precursor Peaks:
-----------------------------
Remove Precursor Peak: True
Mass Window Offset: 4 Da

2. Charge Reduced Precursors:
-----------------------------
Remove Charge Reduced Precursors: True
Mass Window Offset: 2 Da

3. Neutral Losses From Charge Reduced Precursors:
-----------------------------
Remove Neutral Loss Peaks: True
Mass Window Offset: 2 Da
Remove Only Known Masses: True
Maximum Neutral Loss Mass: 120 Da

4. FT Overtones:
-----------------------------
Remove Precursor Overtones: False
Mass Window Offset: 0.5 Da

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Processing node 3: Mascot
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1. Input Data:
-----------------------------
Protein Database: TAIR10
Enzyme Name: Trypsin
Maximum Missed Cleavage Sites: 2
Instrument: ETD-TRAP
Taxonomy: . . . . . . Arabidopsis thaliana (thale cress)

1.1 Peptide Scoring Options:
-----------------------------
Peptide Cut Off Score: 10
Peptide Without Protein Cut Off Score: 5

1.2 Protein Scoring Options:
-----------------------------
Use MudPIT Scoring: Automatic
Protein Relevance Threshold: 20
Protein Relevance Factor: 1

2. Tolerances:
-----------------------------
Precursor Mass Tolerance: 5 ppm
Fragment Mass Tolerance: 0.6 Da
Use Average Precursor Mass: False

3. Dynamic Modifications:
-----------------------------
1. Dynamic Modification: Phosphogood (ST)
2. Dynamic Modification: Phospho (Y)

4. Static Modifications:
-----------------------------
1. Static Modification: Methylthio (C)

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Processing node 4: Percolator
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1. Input Data:
-----------------------------
Maximum Delta Cn: 0.05

2. Decoy Database Search:
-----------------------------
Target FDR (Strict): 0.01
Target FDR (Relaxed): 0.05
Validation based on: q-Value

================================================================================

Processing details:

09/13/2013 10:19 AM (3):Mascot: Total search time was 1 min 37 s.
09/13/2013 10:19 AM (4):Percolator: Performing percolator for Mascot (3) took 29.2 s.
09/13/2013 10:19 AM (3):Mascot: Search completed
09/13/2013 10:19 AM (3):Mascot: Protein grouping (99 groups) took: 0.15 s
09/13/2013 10:19 AM (3):Mascot: 161 protein(s) + 0 decoy proteins scored and inserted into result file in 0.75 s
09/13/2013 10:19 AM (3):Mascot: 161 protein(s) scored
09/13/2013 10:19 AM (3):Mascot: Result finalization started
09/13/2013 10:19 AM (4):Percolator: Start reading Percolator results
09/13/2013 10:18 AM (4):Percolator: Merging results from 3 datasets
09/13/2013 10:18 AM (4):Percolator: Found 274 target PSMs scoring over 1% FDR level on testset
09/13/2013 10:18 AM (4):Percolator: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
09/13/2013 10:18 AM (4):Percolator: 3.39 1.02 9.31 1.09 2.39 -0.355 0.402 -1.55 -0.0842 -1.7 0 -0.118 0.118 0 0 0 -2.28 0 1.51 0.234 0.0713 -1.24 -3.1 -1.44 0.647 -0.52 1.15 -1.48 -2.46 1.59 -0.663 0.185 -5.61
09/13/2013 10:18 AM (4):Percolator: IonScore Delta Cn From Second PSM Binomial Score Isolation Interference [%] MH+ [Da] Delta Mass [Da] Delta Mass [ppm] Absolute Delta Mass [Da] Absolute Delta Mass [ppm] Peptide Length Is z=1 Is z=2 Is z=3 Is z=4 Is z=5 Is z>5 # Missed Cleavages Log Peptides Matched Log Total Intensity Fraction Matched Intensity [%] Fragment Coverage Series A, B, C [%] Fragment Coverage Series X, Y, Z [%] Log Matched Fragment Series Intensities A, B, C Log Matched Fragment Series Intensities X, Y, Z Longest Sequence Series A, B, C Longest Sequence Series X, Y, Z IQR Fragment Delta Mass [Da] IQR Fragment Delta Mass [ppm] Mean Fragment Delta Mass [Da] Mean Fragment Delta Mass [ppm] Mean Absolute Fragment Delta Mass [Da] Mean Absolute Fragment Delta Mass [ppm] m0
09/13/2013 10:18 AM (4):Percolator: # first line contains normalized weights, second line the raw weights
09/13/2013 10:18 AM (4):Percolator: Obtained weights (only showing weights of first cross validation set)
09/13/2013 10:18 AM (4):Percolator: Iteration 10 : After the iteration step, 410 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 9 : After the iteration step, 410 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 8 : After the iteration step, 410 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 7 : After the iteration step, 410 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 6 : After the iteration step, 410 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 5 : After the iteration step, 409 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 4 : After the iteration step, 403 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 3 : After the iteration step, 397 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 2 : After the iteration step, 382 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: Iteration 1 : After the iteration step, 355 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:18 AM (4):Percolator: ---Training with Cpos selected by cross validation, Cneg selected by cross validation, fdr=0.01
09/13/2013 10:18 AM (4):Percolator: Reading in data and feature calculation took 4.703 cpu seconds or 5 seconds wall time
09/13/2013 10:18 AM (4):Percolator: Estimating 185 over q=0.01 in initial direction
09/13/2013 10:18 AM (4):Percolator: A
09/13/2013 10:18 AM (4):Percolator: selecting cneg by cross validation
09/13/2013 10:18 AM (4):Percolator: selecting cpos by cross validation
09/13/2013 10:18 AM (4):Percolator: baFeatureNames::getNumFeatures=32
09/13/2013 10:18 AM (4):Percolator: Train/test set contains 1248 positives and 1174 negatives, size ratio=1.06303 and pi0=1
09/13/2013 10:18 AM (4):Percolator: e6e22773-e9a6-4a26-9694-1ca77a797099 Delta Cn From Second PSM Binomial Score b8754504-e95e-476b-b9a4-454d4bb53aeb 1d91a87b-953a-4887-9f22-f75a497a3538 Delta Mass [Da] Delta Mass [ppm] Absolute Delta Mass [Da] Absolute Delta Mass [ppm] Peptide Length Is z=1 Is z=2 Is z=3 Is z=4 Is z=5 Is z>5 041eb6d5-e486-44a0-9bc1-19e25811c686 Log Peptides Matched Log Total Intensity Fraction Matched Intensity [%] Fragment Coverage Series A, B, C [%] Fragment Coverage Series X, Y, Z [%] Log Matched Fragment Series Intensities A, B, C Log Matched Fragment Series Intensities X, Y, Z Longest Sequence Series A, B, C Longest Sequence Series X, Y, Z IQR Fragment Delta Mass [Da] IQR Fragment Delta Mass [ppm] Mean Fragment Delta Mass [Da] Mean Fragment Delta Mass [ppm] Mean Absolute Fragment Delta Mass [Da] Mean Absolute Fragment Delta Mass [ppm] end of FeatureNames::setFromXml
09/13/2013 10:18 AM (4):Percolator: in FeatureNames::setFromXml
09/13/2013 10:18 AM (4):Percolator: enzyme=Trypsin
09/13/2013 10:18 AM (4):Percolator: Hyperparameters fdr=0.01, Cpos=0, Cneg=0, maxNiter=10
09/13/2013 10:18 AM (4):Percolator: Started Fri Sep 13 10:18:47 2013 on unknown_host
09/13/2013 10:18 AM (4):Percolator: C:\Program Files\Thermo\Discoverer 1.3\Tools\Percolator\percolator.exe -E C:\Documents and Settings\All Users\Application Data\Thermo\Discoverer 1.3\Scratch\97b3808b-01a1-4900-8427-33b55676302f\input.xml -X C:\Documents and Settings\All Users\Application Data\Thermo\Discoverer 1.3\Scratch\97b3808b-01a1-4900-8427-33b55676302f\output.xml -Z
09/13/2013 10:18 AM (4):Percolator: Issued command:
09/13/2013 10:18 AM (4):Percolator: Department of Genome Sciences at the University of Washington.
09/13/2013 10:18 AM (4):Percolator: Written by Lukas K+ñll (lukall@u.washington.edu) in the
09/13/2013 10:18 AM (4):Percolator: Copyright (c) 2006-9 University of Washington. All rights reserved.
09/13/2013 10:18 AM (4):Percolator: Percolator version 1.17, Build Date Nov 30 2010 15:27:43
09/13/2013 10:18 AM (4):Percolator: Starting Percolator
09/13/2013 10:18 AM (4):Percolator: The input file contains 1248 peptides, 1174 decoy peptides and 32 features.
09/13/2013 10:18 AM (4):Percolator: Creating input file for Mascot (3) took 14.1 s.
09/13/2013 10:18 AM (4):Percolator: Start calculating features for peptides of Mascot (3)
09/13/2013 10:18 AM (3):Mascot: Used mascot server http://194.199.86.179/mascot/ with Mascot version 2.2.7
09/13/2013 10:18 AM (3):Mascot: Sending 818 peptide hits (3671 peptides) to result file
09/13/2013 10:18 AM (3):Mascot: Sending 663 decoy peptide hits (3243 peptides) to result file
09/13/2013 10:18 AM (3):Mascot: Reading decoy results
09/13/2013 10:18 AM (3):Mascot: Start translating results
09/13/2013 10:18 AM (3):Mascot: Start mapping modifications
09/13/2013 10:18 AM (3):Mascot: Received 193 proteins from Mascot server
09/13/2013 10:18 AM (3):Mascot: Start mapping 193 proteins
09/13/2013 10:18 AM (3):Mascot: Start parsing results
09/13/2013 10:18 AM (3):Mascot: Received Mascot result file (filename=../data/20130913/F008073.dat)
09/13/2013 10:18 AM (3):Mascot: Mascot Server completed
09/13/2013 10:17 AM (3):Mascot: Mascot result on server (filename=../data/20130913/F008073.dat)
09/13/2013 10:16 AM (3):Mascot: Start searching 3799 spectra
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 799 spectra to Mascot
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 799 spectra to result file
09/13/2013 10:16 AM (2):Non-Fragment Filter: Receiving spectra 3001 to 3800.
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to Mascot
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to result file
09/13/2013 10:16 AM (2):Non-Fragment Filter: Receiving spectra 2001 to 3001.
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to Mascot
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to result file
09/13/2013 10:16 AM (2):Non-Fragment Filter: Receiving spectra 1001 to 2001.
09/13/2013 10:16 AM (3):Mascot: Use mascot server http://194.199.86.179/mascot/ with Mascot version 2.2.7
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to Mascot
09/13/2013 10:16 AM (2):Non-Fragment Filter: Sending 1000 spectra to result file
09/13/2013 10:16 AM (2):Non-Fragment Filter: Receiving spectra 1 to 1001.
09/13/2013 10:16 AM (1):Spectrum Selector: Reading from File 1 of 1:F:\Cytosol\M15F15 022012\Raw files\F_02_23_E1.raw (9187 spectra total)

Top

Confidence	Sequence	Activation Type	Modifications	IonScore	ΔScore	Rank	Search Engine	Charge	Precursor m/z [Da]	ΔM [ppm]	First Scan	Last Scan	Annotated Spectrum	Peak List


High	IIGVDFNsKR	ETD	S8(Phosp)	23.24	1.00	1	Mascot (3)	3	410.20850	1.88	1218	1218	Image	Peak List

High	ENsGFGFLLTR	ETD	S3(Phosp)	28.22	1.00	1	Mascot (3)	2	660.80298	0.17	3110	3110	Image	Peak List

High	ALGSFGsFGsFR	ETD	S7(Phosp); S10(Phosp)	33.09	0.15	1	Mascot (3)	2	696.77356	0.91	3484	3484	Image	Peak List

High	RPFIHtGSWYR	ETD	T6(Phosp)	31.21	0.03	1	Mascot (3)	3	500.57007	0.83	1104	1104	Image	Peak List

Medium	RVSsFEALQPATR	ETD	S4(Phosp)	23.11	0.26	1	Mascot (3)	3	514.58722	-0.10	1092	1092	Image	Peak List

High	KDsFSDLLLHLPR	ETD	S3(Phosp)	16.90	1.00	1	Mascot (3)	3	540.94366	1.13	2655	2655	Image	Peak List

High	LKPFGcNLLYHDR	ETD	C6(Methylthio)	23.35	1.00	1	Mascot (3)	3	541.27289	3.88	1869	1869	Image	Peak List

High	VQsLAESNLSSLPDR	ETD	S3(Phosp)	42.13	1.00	1	Mascot (3)	2	848.40137	0.27	1764	1764	Image	Peak List

High	TRsFDDSPPAAAELR	ETD	S3(Phosp)	18.67	1.00	1	Mascot (3)	2	856.88892	3.80	1094	1094	Image	Peak List

High	NLsGKAETMSTNVER	ETD	S3(Phosp)	59.43	0.56	1	Mascot (3)	3	572.92639	1.34	642	642	Image	Peak List

High	YGAGIGPGVYDIHsPR	ETD	S14(Phosp)	32.17	1.00	1	Mascot (3)	3	580.26965	-0.30	1461	1461	Image	Peak List

High	LStPKPLPSDLLHLK	ETD	T3(Phosp)	26.41	0.27	1	Mascot (3)	3	580.32336	2.97	1927	1927	Image	Peak List

High	APMIIHHADsELMGR	ETD	S10(Phosp)	50.28	1.00	1	Mascot (3)	3	586.60028	-0.05	1169	1169	Image	Peak List

High	SAsFKEESDFFADLK	ETD	S3(Phosp)	17.57	0.11	1	Mascot (3)	2	900.89093	0.95	2609	2609	Image	Peak List

High	FSYNSYPDsAESsPR	ETD	S9(Phosp); S13(Phosp)	17.52	1.00	1	Mascot (3)	2	933.83484	0.23	1568	1568	Image	Peak List

Low	VFcsQVSNLStEMAR	ETD	C3(Methylthio); S4(Phosp); T11(Phosp)	15.78	1.00	1	Mascot (3)	2	939.35858	3.47	2914	2914	Image	Peak List

High	INsAEsMELWASQQK	ETD	S3(Phosp); S6(Phosp)	20.87	1.00	1	Mascot (3)	2	941.38007	2.02	2541	2541	Image	Peak List

High	LIEEVSHSSGsPNPVsD	ETD	S11(Phosp); S16(Phosp)	19.47	1.00	1	Mascot (3)	2	957.38245	0.51	1307	1307	Image	Peak List

High	VTSESDFMtEyVVTR	ETD	T9(Phosp); Y11(Phospho)	32.83	0.24	1	Mascot (3)	2	962.37994	2.20	2401	2401	Image	Peak List

High	VKEGDHIIIGQcRPLSK	ETD	C12(Methylthio)	21.85	1.00	1	Mascot (3)	3	647.01355	0.41	950	950	Image	Peak List

High	VPEAQITNSAtPTTtPR	ETD	T11(Phosp); T15(Phosp)	21.62	0.09	1	Mascot (3)	2	972.43201	2.85	1027	1027	Image	Peak List

High	KTEIVKPEScsNEGDVK	ETD	C10(Methylthio); S11(Phosp)	20.21	0.10	1	Mascot (3)	3	663.62933	1.45	553	553	Image	Peak List

High	TIEKEIsDDEEEEEKK	ETD	S7(Phosp)	27.16	1.00	1	Mascot (3)	3	677.62823	0.55	632	632	Image	Peak List

High	AVGsHcSLSEMDDLDLTR	ETD	S4(Phosp); C6(Methylthio)	25.87	1.00	1	Mascot (3)	2	1037.91809	0.41	2546	2546	Image	Peak List

High	HLQEIPRsPSASPDNGIAK	ETD	S8(Phosp)	21.88	1.00	1	Mascot (3)	3	699.67578	0.11	607	607	Image	Peak List

High	FGIVEGLMTTVHSItATQK	ETD	T15(Phosp)	79.63	0.05	1	Mascot (3)	3	705.01837	0.26	3418	3418	Image	Peak List

High	ADADDDKLVDGLNWAcGQGR	ETD	C16(Methylthio)	32.21	1.00	1	Mascot (3)	3	722.31854	1.07	2711	2711	Image	Peak List

High	KKEEsEEEEGDFGFDLFG	ETD	S5(Phosp)	72.79	1.00	1	Mascot (3)	3	724.62903	1.45	3066	3066	Image	Peak List

High	VSGsEHGFSLNEPLGISENK	ETD	S4(Phosp)	54.40	0.26	1	Mascot (3)	3	727.66754	0.92	1995	1995	Image	Peak List

High	SAIPDTRPRtPIHESAATGR	ETD	T10(Phosp)	19.12	0.16	1	Mascot (3)	3	738.36908	4.66	328	328	Image	Peak List

High	LKTESSsADNIQEDDGSSLR	ETD	S7(Phosp)	24.81	0.32	1	Mascot (3)	2	1116.48950	2.36	674	674	Image	Peak List

High	ANLsPAGDKANVEEPMVYATK	ETD	S4(Phosp)	20.95	1.00	1	Mascot (3)	3	762.35614	0.79	1449	1449	Image	Peak List

High	QQFESIARPTLEIEIPsPR	ETD	S17(Phosp)	23.20	1.00	1	Mascot (3)	3	764.38818	2.95	2536	2536	Image	Peak List

High	IGIWQYGDIEsDDEDTGPAR	ETD	S11(Phosp)	39.70	0.03	1	Mascot (3)	3	772.99475	1.71	2816	2816	Image	Peak List

High	EANVDGDTAsQHEAAWLPGKR	ETD	S10(Phosp)	20.21	1.00	1	Mascot (3)	3	778.01825	2.02	1029	1029	Image	Peak List

High	ATGAFILTAsHNPGGPTEDFGIK	ETD	S10(Phosp)	47.57	0.17	1	Mascot (3)	3	794.37500	-2.93	2567	2567	Image	Peak List

Low	STPGSPAHPPGARsPPPSYLSNK	ETD	S14(Phosp)	17.85	1.00	1	Mascot (3)	3	794.71356	0.94	544	544	Image	Peak List

High	ETLESALLKsPsPDNNNVSEK	ETD	S10(Phosp); S12(Phosp)	30.39	1.00	1	Mascot (3)	3	811.36023	1.26	1997	1997	Image	Peak List

High	SsDSLSGTNELLNINSETPMKK	ETD	S2(Phosp)	47.74	0.17	1	Mascot (3)	3	815.71478	3.36	2074	2074	Image	Peak List

High	GLGDSSSSLKPSSsHTQVAFESIV	ETD	S14(Phosp)	21.43	0.19	1	Mascot (3)	3	834.05933	1.21	2366	2366	Image	Peak List

High	SFDREsPSNLDSTSGISQPHNR	ETD	S6(Phosp)	52.97	0.17	1	Mascot (3)	3	837.69995	-1.75	751	751	Image	Peak List

High	KGYTsDEELEELDSPLTSIVDK	ETD	S5(Phosp)	38.72	0.15	1	Mascot (3)	3	850.05713	-0.88	3012	3012	Image	Peak List

High	VTYVAPPRPPsPVHEGSEEGSSPR	ETD	S11(Phosp)	16.68	1.00	1	Mascot (3)	3	871.41162	2.35	915	915	Image	Peak List

High	HPQWQSDDGGDNsEPESPSDSLR	ETD	S13(Phosp)	40.33	0.45	1	Mascot (3)	3	874.01202	-0.22	961	961	Image	Peak List

High	LVPHTVASQSEVDVAsPVsEKAPK	ETD	S16(Phosp); S19(Phosp)	21.89	1.00	1	Mascot (3)	3	879.08606	0.72	1115	1115	Image	Peak List

High	TSGLPSQTSGAGSAYATLPQLPLsPR	ETD	S24(Phosp)	31.87	1.00	1	Mascot (3)	3	879.77216	3.72	2784	2784	Image	Peak List

High	TLPVAVVEVVKPEsPVLVIVEKPK	ETD	S14(Phosp)	18.92	1.00	1	Mascot (3)	3	883.84851	2.50	2744	2744	Image	Peak List

High	RDDsFQKVNDHPISTVESNLSAK	ETD	S4(Phosp)	28.01	1.00	1	Mascot (3)	3	889.75226	0.41	1246	1246	Image	Peak List

High	VHDMVVADADQDDGtDDDNDLGEK	ETD	T15(Phosp)	40.61	1.00	1	Mascot (3)	3	890.34564	1.11	1153	1153	Image	Peak List

High	KQPEEENGELsEsEKDYDLLPK	ETD	S11(Phosp); S13(Phosp)	32.54	1.00	1	Mascot (3)	3	913.05725	1.53	1617	1617	Image	Peak List

High	EILQSEsFKEEGYLASELQEAEK	ETD	S7(Phosp)	28.85	0.21	1	Mascot (3)	3	913.08917	1.34	2700	2700	Image	Peak List

High	SPTVVTVQPSsPRFPIsTPTAGAQR	ETD	S11(Phosp); S17(Phosp)	16.04	1.00	1	Mascot (3)	3	914.44250	2.52	2119	2119	Image	Peak List

High	FPDDKDGEDEKSGDsGGENLMDSDK	ETD	S15(Phosp)	54.46	0.22	1	Mascot (3)	3	923.02539	1.96	877	877	Image	Peak List

High	VVYVAPPRPPsPVREGsEEGSsPR	ETD	S11(Phosp); S17(Phosp); S22(Phosp)	17.65	0.06	1	Mascot (3)	3	930.40955	1.54	1491	1491	Image	Peak List

High	KPEDGsAVAAAPVVVPPPVEEAHPVEK	ETD	S6(Phosp)	20.44	1.00	1	Mascot (3)	3	933.80414	0.45	1435	1435	Image	Peak List

High	AKETGPEIETAVDGEsVHEIETTER	ETD	S16(Phosp)	17.18	1.00	1	Mascot (3)	3	936.42657	1.13	1335	1335	Image	Peak List

Medium	KSPTVVTVQPSsPRFPIsTPTAGAQR	ETD	S12(Phosp); S18(Phosp)	24.63	1.00	1	Mascot (3)	3	957.13702	-1.57	1703	1703	Image	Peak List

High	ISHSTGSETLDQNMVLGEVsPKDEESR	ETD	S20(Phosp)	15.70	1.00	1	Mascot (3)	3	1009.12085	0.74	1430	1430	Image	Peak List

High	LSKsDDSLGSQFLMSHPQASTGQQEPAK	ETD	S4(Phosp)	25.22	0.16	1	Mascot (3)	3	1018.80188	1.16	1519	1519	Image	Peak List

Medium	EKPSSPPtFDDGPtSPPASLHEPEPSAK	ETD	T8(Phosp); T14(Phosp)	19.83	1.00	1	Mascot (3)	3	1021.43561	-4.78	1426	1426	Image	Peak List

High	WDsTTSEEAKENLIFQSGGDREEVDR	ETD	S3(Phosp)	25.78	0.04	1	Mascot (3)	3	1026.78687	4.38	1736	1736	Image	Peak List

High	ASLsTGNIPEMFDGRPSFTEGSGLEASPR	ETD	S4(Phosp)	15.65	1.00	1	Mascot (3)	3	1030.80054	-0.17	2779	2779	Image	Peak List

High	GMVSSGGPVsPGPVYPGGRPGAGGLMPGMPGTR	ETD	S10(Phosp)	24.79	0.36	1	Mascot (3)	3	1039.82373	3.09	2284	2284	Image	Peak List

High	NVEGsNEDEEEEIEEDGEEISEVDHAK	ETD	S5(Phosp)	15.68	1.00	1	Mascot (3)	3	1047.41138	-0.92	2207	2207	Image	Peak List

High	SSIQDDEsNAEETDDQHLASDHATDITR	ETD	S8(Phosp)	19.65	1.00	1	Mascot (3)	3	1060.77161	4.04	931	931	Image	Peak List

High	STsSNPADDIPLTEAKDEEEASEVAGLEAK	ETD	S3(Phosp)	21.56	0.05	1	Mascot (3)	3	1061.81018	1.81	2483	2483	Image	Peak List

Medium	KFSEQNIGAPPSYEEAVSDSRsPVYSER	ETD	S22(Phosp)	23.78	0.25	1	Mascot (3)	3	1070.49084	4.85	1570	1570	Image	Peak List

High	RKGDGDDEEsEDESAENVVNVEDFLVQK	ETD	S10(Phosp)	54.21	0.67	1	Mascot (3)	3	1078.13306	1.33	2597	2597	Image	Peak List

High	ASEAEHEDPVDDIKsNDDRDFPTEQAPK	ETD	S15(Phosp)	17.83	1.00	1	Mascot (3)	3	1079.13342	4.83	1097	1097	Image	Peak List

High	IISQLEPEVLsPIKPADDQLSLSDLDMVK	ETD	S11(Phosp)	54.10	0.20	1	Mascot (3)	3	1091.89343	2.45	3472	3472	Image	Peak List

High	KTDGSTtPAYAHGQHHSIFSPATGAVSDSSLK	ETD	T7(Phosp)	22.60	0.17	1	Mascot (3)	3	1112.51575	-0.08	980	980	Image	Peak List

High	HDSSSSyKSDADDDVDFSETTSNPTWLEK	ETD	Y7(Phospho)	37.58	0.05	1	Mascot (3)	3	1115.12073	1.22	1916	1916	Image	Peak List

High	KEEATPAPAVVEtPVKEPETTTTAPVAEPPKP	ETD	T13(Phosp)	20.65	1.00	1	Mascot (3)	3	1131.23413	-1.29	1286	1286	Image	Peak List

High	sPEHALFTKPVYDQTEQLPPAPWETQEPR	ETD	S1(Phosp)	18.50	0.06	1	Mascot (3)	3	1157.88220	1.80	2513	2513	Image	Peak List

High	EISHDSsDEEDGDGGKNtNEMLNYGLTIASK	ETD	S7(Phosp); T18(Phosp)	17.07	1.00	1	Mascot (3)	3	1162.80554	2.62	2480	2480	Image	Peak List

High	EATADMSEEFSEGEKGDIIsDISTHGESTKPR	ETD	S20(Phosp)	32.43	0.19	1	Mascot (3)	3	1178.51550	1.59	1897	1897	Image	Peak List

High	FNNHsPEENNNSPLSGSSATNTNETELSLMLK	ETD	S5(Phosp)	19.44	1.00	1	Mascot (3)	3	1186.19507	4.82	2422	2422	Image	Peak List

High	EATADMsEEFsEGEKGDIISDISTHGESTKPR	ETD	S7(Phosp); S11(Phosp)	16.64	1.00	1	Mascot (3)	3	1205.17126	1.82	2105	2105	Image	Peak List

Low	SSAVQEEQFGEHTEPcSSEIKDESHGKEESVEVK	ETD	C16(Methylthio)	22.14	1.00	1	Mascot (3)	3	1274.56030	-2.38	1073	1073	Image	Peak List

Low	SKDNLYEQKPEEPVPVIPAASPtNDTSAAGSSFASR	ETD	T23(Phosp)	21.20		2	Mascot (3)	3	1280.94006	2.37	1825	1825	Image	Peak List

High	IDLDKPEVEDDDDNDEDDsEDDDEAEGHDGEAGGR	ETD	S19(Phosp)	55.75	1.00	1	Mascot (3)	3	1304.80688	-1.54	1024	1024	Image	Peak List

Low	QDcPLINIEVVAHVTSPEIRssEVsNtFyFK	ETD	C3(Methylthio); S21(Phosp); S22(Phosp); S25(Phosp); T27(Phosp); Y29(Phospho)	21.43	1.00	1	Mascot (3)	3	1323.52808	-1.81	1500	1500	Image	Peak List

High	DAETVNQTSHPTEEEAQVTVSSNADVEDSHETVsPR	ETD	S34(Phosp)	28.64	0.21	1	Mascot (3)	3	1325.90588	0.75	1125	1125	Image	Peak List

Low	sDPFFFVsGNsGScLKGQKLAVTVMSTGHHSHtPR	ETD	S1(Phosp); S8(Phosp); S11(Phosp); C14(Methylthio); T33(Phosp)	17.21	1.00	1	Mascot (3)	3	1361.55688	-0.77	95	95	Image	Peak List

Annotated spectra of F_02_23_E1_ETD.msf

Peptides

Search Summary