Annotated spectra of F_03_23_E2_MSA.msf

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Peptides

Confidence Sequence Activation Type Modifications IonScore ΔScore Rank Search Engine Charge Precursor m/z [Da] ΔM [ppm] First Scan Last Scan Annotated Spectrum Peak List
High FAMPLsR CID S6(PhospR4) 18.42 1.00 1 Mascot (2) 2 451.20227 -3.26 2360 2360 Image Peak List
High SMsFSPDRVR CID S3(PhospR4) 25.53 0.08 1 Mascot (2) 2 631.27289 -0.84 1002 1002 Image Peak List
High KITtQPINIR CID T4(PhospR4) 32.27 0.19 1 Mascot (2) 2 632.34570 1.60 920 920 Image Peak List
High NcKsLENMVK CID C2(Methylthio); S4(PhospR4) 30.04 1.00 1 Mascot (2) 2 646.26703 1.76 1513 1513 Image Peak List
High GEPNISyIcSR CID Y7(Phospho); C9(Methylthio) 43.55 0.25 1 Mascot (2) 2 682.77307 1.01 2554 2554 Image Peak List
High SAsVGNWILEPK CID S3(PhospR4) 44.92 0.13 1 Mascot (2) 2 690.83246 1.21 2558 2558 Image Peak List
High ALGsFGSFGsFR CID S4(PhospR4); S10(PhospR4) 42.44 0.14 1 Mascot (2) 2 696.77350 0.82 3466 3466 Image Peak List
High NNsLLSGIIDGGR CID S3(PhospR4) 63.49 0.52 1 Mascot (2) 2 698.33582 1.08 2866 2866 Image Peak List
High SFsGGEcADWSK CID S3(PhospR4); C7(Methylthio) 22.24 1.00 1 Mascot (2) 2 700.23901 0.90 2243 2243 Image Peak List
High AFFDsADWALGK CID S5(PhospR4) 73.87 1.00 1 Mascot (2) 2 704.30249 -0.30 3207 3207 Image Peak List
High GGSFLAGNLsFNR CID S10(PhospR4) 31.35 0.35 1 Mascot (2) 2 710.32581 1.84 2568 2568 Image Peak List
High DKDDFDDFcLR CID C9(Methylthio) 48.41 1.00 1 Mascot (2) 2 717.78760 0.99 2402 2402 Image Peak List
High VPsPcLtPIGSGR CID S3(PhospR4); C5(Methylthio); T7(PhospR4) 31.49 0.32 1 Mascot (2) 2 745.30444 2.37 2325 2325 Image Peak List
High cVsLNNLSDYEK CID C1(Methylthio); S3(PhospR4) 57.48 1.00 1 Mascot (2) 2 755.80280 1.93 2264 2264 Image Peak List
High VHAFPLsPTSLLR CID S7(PhospR4) 54.53 0.24 1 Mascot (2) 2 759.39838 1.20 2754 2754 Image Peak List
High KIsGILDDGSVGFR CID S3(PhospR4) 72.44 0.75 1 Mascot (2) 2 772.38013 0.84 2071 2071 Image Peak List
High VGVGAEsPAAATDcSK CID S7(PhospR4); C14(Methylthio) 59.91 0.38 1 Mascot (2) 2 794.82312 0.37 1091 1091 Image Peak List
High HPWLSYPYEPIsA CID S12(PhospR4) 29.24 1.00 1 Mascot (2) 2 820.36365 0.45 2955 2955 Image Peak List
High TVQFVDWcPtGFK CID C8(Methylthio); T10(PhospR4) 54.51 1.00 1 Mascot (2) 2 827.34705 1.59 3748 3748 Image Peak List
High NSsPPsPFHPAAYK CID S3(PhospR4); S6(PhospR4) 30.50 1.00 1 Mascot (2) 2 830.33441 0.65 1338 1338 Image Peak List
High SFPGGsGPIRVPscK CID S6(PhospR4); S13(PhospR4); C14(Methylthio) 27.79 0.32 1 Mascot (2) 2 847.84637 1.33 2570 2570 Image Peak List
High SVsVKEEETVVVAEK CID S3(PhospR4) 48.30 0.04 1 Mascot (2) 2 856.92206 0.80 1079 1079 Image Peak List
High NLsGKAETMSTNVER CID S3(PhospR4) 50.57 1.00 1 Mascot (2) 2 858.88489 0.10 645 645 Image Peak List
High sKSELEQcVTEESK CID S1(PhospR4); C8(Methylthio) 45.00 0.02 1 Mascot (2) 2 861.85181 0.04 1347 1347 Image Peak List
High LStPKPLPSDLLHLK CID T3(PhospR4) 28.22 1.00 1 Mascot (2) 3 580.32153 -0.19 1954 1954 Image Peak List
High WIcHDWSDEHcVK CID C3(Methylthio); C11(Methylthio) 20.83 1.00 1 Mascot (2) 3 583.89301 0.20 1772 1772 Image Peak List
High HYAHVDcPGHADYVK CID C7(Methylthio) 37.45 1.00 1 Mascot (2) 2 879.37836 -1.64 493 493 Image Peak List
High HcHSEESNMcDLLR CID C2(Methylthio); C10(Methylthio) 68.73 1.00 1 Mascot (2) 2 883.33386 1.17 1634 1634 Image Peak List
High FTNPsPcNsPVLQR CID S5(PhospR4); C7(Methylthio); S9(PhospR4) 24.25 1.00 1 Mascot (2) 2 883.34717 1.77 2068 2068 Image Peak List
Medium EETKTVPSVAYYsPK CID S13(PhospR4) 18.49 1.00 1 Mascot (2) 3 593.61420 1.78 1863 1863 Image Peak List
High FSAFsPNKYLEEVGK CID S5(PhospR4) 21.98 1.00 1 Mascot (2) 2 898.41980 1.12 2549 2549 Image Peak List
High VFcSQVsNLSTEMAR CID C3(Methylthio); S7(PhospR4) 88.11 0.36 1 Mascot (2) 2 899.37335 1.33 2675 2675 Image Peak List
High VQIESGVMtAETGTYR CID T9(PhospR4) 88.32 0.31 1 Mascot (2) 2 911.40973 1.78 1674 1674 Image Peak List
High KLsSALSAASSAcDHIR CID S3(PhospR4); C13(Methylthio) 60.56 0.05 1 Mascot (2) 3 614.94690 1.23 2108 2108 Image Peak List
High VTSESDFMtEYVVTR CID T9(PhospR4) 59.09 0.36 1 Mascot (2) 2 922.39630 1.78 2442 2442 Image Peak List
High VLsPYSLPASLLHSER CID S3(PhospR4) 23.33 1.00 1 Mascot (2) 3 616.98077 1.17 2806 2806 Image Peak List
High LFcDKDYPEKPPTVR CID C3(Methylthio) 48.16 1.00 1 Mascot (2) 3 618.63641 1.16 1256 1256 Image Peak List
High IVFVTcDDDDKVADIR CID C6(Methylthio) 21.68 1.00 1 Mascot (2) 3 623.96252 0.43 2134 2134 Image Peak List
High TKSETDFMtEYVVTR CID T9(PhospR4) 83.89 0.42 1 Mascot (2) 2 943.91827 2.67 1961 1961 Image Peak List
High ISsVDVFENWFAQHK CID S3(PhospR4) 54.61 0.04 1 Mascot (2) 2 943.92694 -0.40 3114 3114 Image Peak List
High EAFSLFDKDGDGcITTK CID C13(Methylthio) 39.88 1.00 1 Mascot (2) 3 631.61951 1.79 2236 2236 Image Peak List
High TPYHPMMDDDGsLSPR CID S12(PhospR4) 45.14 0.02 1 Mascot (2) 2 949.87799 1.97 1196 1196 Image Peak List
High VTSESDFMtEyVVTR CID T9(PhospR4); Y11(Phospho) 70.47 0.16 1 Mascot (2) 2 962.37921 1.44 2414 2414 Image Peak List
High VKEGDHIIIGQcRPLSK CID C12(Methylthio) 33.82 1.00 1 Mascot (2) 3 647.01422 1.45 951 951 Image Peak List
High ESKsHLNLNEcLTLPK CID S4(PhospR4); C11(Methylthio) 34.86 1.00 1 Mascot (2) 3 651.30579 0.52 2171 2171 Image Peak List
High LELSAVPSsYSSGQLDPK CID S9(PhospR4) 26.88 0.04 1 Mascot (2) 2 979.46545 4.15 2185 2185 Image Peak List
High SIPEGAVVcNVEHHVGDR CID C9(Methylthio) 48.81 1.00 1 Mascot (2) 3 655.30975 1.69 1406 1406 Image Peak List
High TPYHPMMDDDGsLsPR CID S12(PhospR4); S14(PhospR4) 25.81 1.00 1 Mascot (2) 2 989.86072 1.45 1469 1469 Image Peak List
Medium EDDKVALQEDNcADDVR CID C12(Methylthio) 17.06 1.00 1 Mascot (2) 3 660.94781 0.73 1123 1123 Image Peak List
High SGFIHHcLVSDLEHDTK CID C7(Methylthio) 33.31 1.00 1 Mascot (2) 3 661.97369 0.66 1385 1385 Image Peak List
High tNSSPGNFESPLDRPMR CID T1(PhospR4) 24.47 0.04 1 Mascot (2) 2 992.93231 -0.51 1487 1487 Image Peak List
High EEKEEsDDDMGFSLFE CID S6(PhospR4) 23.34 1.00 1 Mascot (2) 2 993.86530 1.70 3398 3398 Image Peak List
High KSMIPQNLGsFKEESSK CID S10(PhospR4) 40.81 0.27 1 Mascot (2) 2 995.47113 -0.11 1284 1284 Image Peak List
High sMSFVGTHEYLAPEIIK CID S1(PhospR4) 47.99 0.33 1 Mascot (2) 2 1001.47479 1.54 2572 2572 Image Peak List
High TIEKEIsDDEEEEEKK CID S7(PhospR4) 31.82 1.00 1 Mascot (2) 3 677.62866 1.18 652 652 Image Peak List
High TLEAPLPGsLsAVNNATTR CID S9(PhospR4); S11(PhospR4) 48.84 0.63 1 Mascot (2) 2 1036.47815 1.37 2724 2724 Image Peak List
High STsGVDGNSPVSVcISPGER CID S3(PhospR4); C14(Methylthio) 72.06 0.04 1 Mascot (2) 2 1037.43738 2.74 2393 2393 Image Peak List
High AVGSHcsLSEMDDLDLTR CID C6(Methylthio); S7(PhospR4) 40.04 0.28 1 Mascot (2) 3 692.28174 1.26 2563 2563 Image Peak List
High LIHPLPPALSsPETsPER CID S11(PhospR4); S15(PhospR4) 29.34 0.14 1 Mascot (2) 3 700.99762 0.74 2082 2082 Image Peak List
High KTsVDDQASQHLLSLLQR CID S3(PhospR4) 55.35 0.13 1 Mascot (2) 3 707.02362 0.90 2229 2229 Image Peak List
High cKHDPSHWDQISLGGLER CID C1(Methylthio) 36.60 1.00 1 Mascot (2) 3 708.66357 0.53 1387 1387 Image Peak List
High ADADDDKLVDGLNWAcGQGR CID C16(Methylthio) 33.70 1.00 1 Mascot (2) 3 722.31866 1.24 2736 2736 Image Peak List
High RsYsPGYEGAAAAAPDRDR CID S2(PhospR4); S4(PhospR4) 28.59 0.21 1 Mascot (2) 3 723.96289 -0.47 682 682 Image Peak List
High GRLELSAVPSSYSsGQLDPK CID S14(PhospR4) 22.05 0.09 1 Mascot (2) 2 1086.02417 1.37 1742 1742 Image Peak List
High DSDDQNAAWNVPLEAcIHK CID C16(Methylthio) 52.44 1.00 1 Mascot (2) 3 724.65643 2.85 2852 2852 Image Peak List
High SIsDASSQSLSSILNNPHGGK CID S3(PhospR4) 61.66 0.03 1 Mascot (2) 2 1090.00708 1.89 1963 1963 Image Peak List
High TSsFRDPLSVSDSASPIPSR CID S3(PhospR4) 31.68 0.13 1 Mascot (2) 2 1093.51208 1.96 1975 1975 Image Peak List
High VLSHAVsESDVSVHSTFASR CID S7(PhospR4) 91.72 0.12 1 Mascot (2) 2 1098.01392 3.47 1105 1105 Image Peak List
High KLEDIVPSSHNcDVPHVNR CID C12(Methylthio) 19.27 1.00 1 Mascot (2) 3 735.69141 2.56 8515 8515 Image Peak List
Medium TTQQPEKPGSVNsQPPPWR CID S13(PhospR4) 17.42 1.00 1 Mascot (2) 3 738.68268 -0.24 890 890 Image Peak List
High FSMDAGAFDAVVcSHHAHSGK CID C13(Methylthio) 26.17 1.00 1 Mascot (2) 3 740.65271 0.69 1793 1793 Image Peak List
High LKTESSsADNIQEDDGSSLR CID S7(PhospR4) 49.64 0.20 1 Mascot (2) 2 1116.48486 -1.79 689 689 Image Peak List
High FLVGVKPADDFLDSHSsPPK CID S17(PhospR4) 26.32 1.00 1 Mascot (2) 3 746.02924 2.00 2073 2073 Image Peak List
High VLSHAVSEsDVsVHSTFASR CID S9(PhospR4); S12(PhospR4) 54.84 0.04 1 Mascot (2) 2 1137.99670 3.02 1289 1289 Image Peak List
High HYSEDVGEVQASQEKPVsPK CID S18(PhospR4) 44.56 0.47 1 Mascot (2) 3 765.35046 1.35 456 456 Image Peak List
High IKFDPSDLTADKEDLPcDVK CID C17(Methylthio) 40.75 1.00 1 Mascot (2) 3 765.70203 1.35 2337 2337 Image Peak List
High cDGGDDIDDVQSHSTDEPVKK CID C1(Methylthio) 36.32 1.00 1 Mascot (2) 3 769.32391 1.02 484 484 Image Peak List
High IGIWQYGDIEsDDEDTGPAR CID S11(PhospR4) 28.17 1.00 1 Mascot (2) 3 772.99408 0.84 2815 2815 Image Peak List
High sMESNVANGNTTIDIHHPcR CID S1(PhospR4); C19(Methylthio) 36.13 0.31 1 Mascot (2) 3 774.65619 3.36 1371 1371 Image Peak List
High AVTDTEsPHLGEITcGTLLEK CID S7(PhospR4); C15(Methylthio) 38.18 0.03 1 Mascot (2) 3 780.68915 1.24 2920 2920 Image Peak List
High QGSGGVWGWLAGSPQEKDDDsP CID S21(PhospR4) 23.62 1.00 1 Mascot (2) 2 1176.99304 0.78 3284 3284 Image Peak List
High KFDWDHPLHLQPMsPTTVK CID S14(PhospR4) 42.96 0.12 1 Mascot (2) 3 786.37732 1.06 2057 2057 Image Peak List
High MTPtKPMVVETFSEYPPLGR CID T4(PhospR4) 31.96 1.00 1 Mascot (2) 3 787.37469 1.19 2600 2600 Image Peak List
High KVEEVKDEssDDAGMMGLFD CID S9(PhospR4); S10(PhospR4) 53.93 1.00 1 Mascot (2) 2 1181.45337 3.80 2904 2904 Image Peak List
High AtGAFILTASHNPGGPTEDFGIK CID T2(PhospR4) 59.23 0.05 1 Mascot (2) 2 1191.06543 2.58 2428 2428 Image Peak List
High AVDAGMLDyDSDDNPIVVDKR CID Y9(Phospho) 41.66 0.12 1 Mascot (2) 3 796.68549 -0.30 2194 2194 Image Peak List
High KFsEQNIGAPPSYEEAVSDSR CID S3(PhospR4) 61.57 1.00 1 Mascot (2) 2 1196.02588 -2.36 1604 1604 Image Peak List
Medium sATIEEDVASISGRPFSDPGSSK CID S1(PhospR4) 19.39 1.00 1 Mascot (2) 3 806.36926 2.61 2526 2526 Image Peak List
High sAPTTPINQNAAAAFAAVSEEER CID S1(PhospR4) 99.96 0.04 1 Mascot (2) 2 1213.05688 1.34 2834 2834 Image Peak List
High HIEDTWMQELNANRPAtPTR CID T17(PhospR4) 22.15 0.18 1 Mascot (2) 3 820.71045 1.67 1891 1891 Image Peak List
High VQEVPHEGPMcDLLWSDPDDR CID C11(Methylthio) 25.40 1.00 1 Mascot (2) 3 828.69360 1.32 2864 2864 Image Peak List
High ANGGFIMSAsHNPGGPEYDWGIK CID S10(PhospR4) 44.54 0.16 1 Mascot (2) 3 829.02673 0.52 2460 2460 Image Peak List
High TVVASIADETVAESSGTGNKSFsR CID S23(PhospR4) 22.31 0.18 1 Mascot (2) 3 831.72217 0.55 1763 1763 Image Peak List
High SAPttPINQNAAAAFAAVSEEER CID T4(PhospR4); T5(PhospR4) 25.91 1.00 1 Mascot (2) 3 835.69574 1.23 3167 3167 Image Peak List
High RQGSGGVWGWLAGSPQEKDDDsP CID S22(PhospR4) 67.52 0.68 1 Mascot (2) 3 837.03223 1.60 2687 2687 Image Peak List
High HSVLHDQETGEEYcPLDHLIK CID C14(Methylthio) 56.50 1.00 1 Mascot (2) 3 837.05493 0.61 1989 1989 Image Peak List
High TKNsNHDENEFISFEPNQNTK CID S4(PhospR4) 20.30 0.05 1 Mascot (2) 3 858.37177 2.58 1291 1291 Image Peak List
High RSsLGLSPPGNNLDELLHGNFDR CID S3(PhospR4) 48.93 0.10 1 Mascot (2) 3 863.41193 -1.62 2684 2684 Image Peak List
High EAPAAEAEKsVSVKEEETVVVAEK CID S10(PhospR4) 46.48 0.33 1 Mascot (2) 3 870.42462 -0.07 1282 1282 Image Peak List
High TYVADVSEYLGSNsPRDPYLER CID S14(PhospR4) 59.78 0.15 1 Mascot (2) 3 871.06354 1.83 2500 2500 Image Peak List
Medium EVISDPStPKPNTEPFAHYPQGK CID T8(PhospR4) 18.56 0.05 1 Mascot (2) 3 873.74048 -2.25 1175 1175 Image Peak List
High AGDStPEELANATQVQGDYLPIVR CID T5(PhospR4) 83.85 0.75 1 Mascot (2) 2 1312.62085 3.94 2934 2934 Image Peak List
High TSGLPSQTSGAGSAYATLPQLPLsPR CID S24(PhospR4) 21.18 1.00 1 Mascot (2) 3 879.76837 -0.58 2773 2773 Image Peak List
High TPEINKAPEVNAPTPVSLsPKPDR CID S19(PhospR4) 21.09 1.00 1 Mascot (2) 3 879.78296 2.21 1331 1331 Image Peak List
High VEEKEEsDEEDYGGDFGLFDEE CID S7(PhospR4) 29.62 1.00 1 Mascot (2) 3 882.99731 0.21 3020 3020 Image Peak List
High APPATLTsPKPPSSSDFSIPTIDLK CID S8(PhospR4) 19.35 1.00 1 Mascot (2) 3 883.11542 1.86 2733 2733 Image Peak List
High TLPVAVVEVVKPEsPVLVIVEKPK CID S14(PhospR4) 50.84 1.00 1 Mascot (2) 3 883.84766 1.53 2764 2764 Image Peak List
High IStPELPPPVFIKPESPEPVSTPK CID T3(PhospR4) 25.35 1.00 1 Mascot (2) 3 889.46283 0.35 2444 2444 Image Peak List
High VHDMVVADADQDDGtDDDNDLGEK CID T15(PhospR4) 33.15 1.00 1 Mascot (2) 3 890.34851 4.34 1193 1193 Image Peak List
High TKDELTEEEsLSGKDYLDPPPVK CID S10(PhospR4) 26.07 0.12 1 Mascot (2) 3 890.75330 0.69 1702 1702 Image Peak List
High MDVNSQNAVLRsPQQLSHLFPGR CID S12(PhospR4) 36.39 0.31 1 Mascot (2) 3 892.10394 1.95 2365 2365 Image Peak List
High ITsSPKQEIGTGEATEQEEGKEQK CID S3(PhospR4) 38.30 1.00 1 Mascot (2) 3 895.41870 4.32 428 428 Image Peak List
High AAAVAALSQVLVAENKKsPDTSPtR CID S18(PhospR4); T24(PhospR4) 20.44 0.05 1 Mascot (2) 3 895.44043 0.37 2208 2208 Image Peak List
High DIQGSDNAIPLsPQWLLSKPGENK CID S12(PhospR4) 42.84 0.35 1 Mascot (2) 3 896.44171 1.05 2943 2943 Image Peak List
High KRSAPtTPINQNAAAAFAAVSEEER CID T6(PhospR4) 106.67 1.00 1 Mascot (2) 3 903.77258 1.43 1917 1917 Image Peak List
High SSDVQAEVFGEHTEPcSSEIKDDR CID C16(Methylthio) 47.22 1.00 1 Mascot (2) 3 904.39130 1.20 1583 1583 Image Peak List
High RPRtPPATPGIVDYQNPDHELMK CID T4(PhospR4) 20.43 1.00 1 Mascot (2) 3 904.77136 1.13 1161 1161 Image Peak List
Medium LQTFSsFQKPLEPPNNNAPPRPR CID S6(PhospR4) 18.28 1.00 1 Mascot (2) 3 905.78943 4.91 1501 1501 Image Peak List
High NILKPADAVTPSTHSSTFFSVQsPR CID S23(PhospR4) 33.06 0.36 1 Mascot (2) 3 923.12073 0.88 1905 1905 Image Peak List
High EHNAVcDALKDEDDDLEDEDLYR CID C6(Methylthio) 67.45 1.00 1 Mascot (2) 3 923.38361 2.29 2409 2409 Image Peak List
High AGDStPEELANATQVQGDYMPIASVK CID T5(PhospR4) 29.79 0.03 1 Mascot (2) 3 924.75427 1.71 2680 2680 Image Peak List
Medium MKLPLDIDsPTQSENSSSSQQtPK CID S9(PhospR4); T22(PhospR4) 17.92 1.00 1 Mascot (2) 3 926.74048 4.57 2388 2388 Image Peak List
High NVAGEEDNDsRAsSISSLLDSSVNR CID S10(PhospR4); S13(PhospR4) 23.02 1.00 1 Mascot (2) 3 928.05627 -0.82 2537 2537 Image Peak List
High VEEKKEEsDEEDYEGGFGLFDEE CID S8(PhospR4) 42.91 0.58 1 Mascot (2) 3 930.36884 1.61 2565 2565 Image Peak List
High VVYVAPPRPPsPVREGSEEGssPR CID S11(PhospR4); S21(PhospR4); S22(PhospR4) 29.35 1.00 1 Mascot (2) 3 930.40948 1.47 1532 1532 Image Peak List
High KRSAPttPINQNAAAAFAAVSEEER CID T6(PhospR4); T7(PhospR4) 95.23 0.02 1 Mascot (2) 3 930.42462 -2.28 2103 2103 Image Peak List
High RANsYHSYSQSPPyDYQYEER CID S4(PhospR4); Y14(Phospho) 24.48 0.17 1 Mascot (2) 3 934.02948 0.93 1172 1172 Image Peak List
High LHTVPEVAEEEGDKDEYHDAsDKQ CID S21(PhospR4) 19.05 1.00 1 Mascot (2) 3 941.06720 1.17 605 605 Image Peak List
Medium IsTPELPPPVFIKPEsPEPVStPK CID S2(PhospR4); S16(PhospR4); T22(PhospR4) 18.52 1.00 1 Mascot (2) 3 942.77167 -1.84 3412 3412 Image Peak List
High LAPAEAVVEDNQKAsDIEEGDEVDSK CID S15(PhospR4) 20.38 1.00 1 Mascot (2) 3 946.75739 0.35 1438 1438 Image Peak List
High KPHTSTADLLTWSEVPPPDsPSSASR CID S20(PhospR4) 24.18 0.25 1 Mascot (2) 3 948.44922 2.90 2017 2017 Image Peak List
High LPLDIDsPTKSENSSSDSQKLPtPK CID S7(PhospR4); T23(PhospR4) 20.99 1.00 1 Mascot (2) 3 948.77045 0.53 1627 1627 Image Peak List
High FPDDKDGEDEKsGDsGGENLMDSDK CID S12(PhospR4); S15(PhospR4) 18.65 1.00 1 Mascot (2) 3 949.68109 2.18 1165 1165 Image Peak List
High FEDQSETsSSHGGITAPGPISVAQLPR CID S8(PhospR4) 37.99 0.03 1 Mascot (2) 3 950.11206 2.32 2176 2176 Image Peak List
Medium IGYVLQQPGLAAMPQPPPRHsPPYR CID S21(PhospR4) 17.93 1.00 1 Mascot (2) 3 950.81525 1.32 1907 1907 Image Peak List
High VKAGDStPEELANATQVQGDYLPIVR CID T7(PhospR4) 20.72 0.05 1 Mascot (2) 3 951.13611 2.21 2481 2481 Image Peak List
High ERPRsPPTNSLSMDYQTADSESVLK CID S5(PhospR4) 21.41 1.00 1 Mascot (2) 3 963.44397 1.40 1520 1520 Image Peak List
High LGQLSIPQESSPcsPVTKPGSSGASLSR CID C13(Methylthio); S14(PhospR4) 34.39 0.21 1 Mascot (2) 3 966.12500 0.79 2213 2213 Image Peak List
High DLEsPTVSTAHTGAAGGGAASGVTHLGVVGR CID S4(PhospR4) 79.59 0.11 1 Mascot (2) 3 971.46881 0.66 1480 1480 Image Peak List
High DLSMNKFDWDHPLHLQPMsPTTVK CID S19(PhospR4) 30.21 0.03 1 Mascot (2) 3 973.11987 1.26 2579 2579 Image Peak List
High ASDNSVSASSSTAEREsDEDSSNALAVR CID S17(PhospR4) 47.73 0.17 1 Mascot (2) 3 974.74750 1.56 780 780 Image Peak List
High ITLLEELQEKtEEDEENKPSVIEK CID T11(PhospR4) 44.77 0.40 1 Mascot (2) 3 975.14148 0.89 2059 2059 Image Peak List
High REDQEPGLQtPDLILsEVEDEKPK CID T10(PhospR4); S16(PhospR4) 42.98 1.00 1 Mascot (2) 3 975.77905 2.00 2759 2759 Image Peak List
High FEDQSEtsSSHGGITAPGPISVAQLPR CID T7(PhospR4); S8(PhospR4) 30.18 0.03 1 Mascot (2) 3 976.76813 2.89 2556 2556 Image Peak List
Medium SLPLHIEDSASLPQQEPNSNssGEIK CID S21(PhospR4); S22(PhospR4) 18.27 1.00 1 Mascot (2) 3 979.77039 1.71 2255 2255 Image Peak List
High DWLESQGHQYIVTDDKEGPDcELEK CID C21(Methylthio) 66.13 1.00 1 Mascot (2) 3 994.10553 1.47 2141 2141 Image Peak List
High KPEDSQVVNTTPLVETAtPIADIPEEK CID T18(PhospR4) 45.36 0.02 1 Mascot (2) 3 1001.16150 1.49 2192 2192 Image Peak List
High AFsPSIVSGSQWRPGSPFQSQNEtVR CID S3(PhospR4); T24(PhospR4) 21.86 1.00 1 Mascot (2) 3 1003.78204 0.75 2740 2740 Image Peak List
High ISHSTGSETLDQNMVLGEVsPKDEESR CID S20(PhospR4) 63.39 0.40 1 Mascot (2) 3 1009.12244 2.32 1450 1450 Image Peak List
High VAsDTYREPHQSPYNVAQPLQAPIGTK CID S3(PhospR4) 32.75 0.18 1 Mascot (2) 3 1016.49518 2.83 1506 1506 Image Peak List
High TPNIEPQGYsEEEEEEEEEVPAGNAAK CID S10(PhospR4) 32.30 0.25 1 Mascot (2) 3 1019.08929 -1.43 1709 1709 Image Peak List
High TTPASASASyFHTMSsDDEDEKEVHR CID Y10(Phospho); S16(PhospR4) 40.04 0.18 1 Mascot (2) 3 1020.06750 2.26 1198 1198 Image Peak List
High VHDDDEDVssEDENETHNSNAVYYK CID S9(PhospR4); S10(PhospR4) 42.89 0.51 1 Mascot (2) 3 1024.37805 3.12 755 755 Image Peak List
High FSSLEKPQFDHQNSSGsNYDDLTPHR CID S17(PhospR4) 31.41 0.16 1 Mascot (2) 3 1029.45215 4.60 1235 1235 Image Peak List
High IEEEsENEEEEEGEEEDDDEEVKEK CID S5(PhospR4) 102.13 1.00 1 Mascot (2) 3 1036.05347 0.95 601 601 Image Peak List
High LQNNDDMDASPSQPLSQsFDPcFNTSK CID S18(PhospR4); C22(Methylthio) 34.31 0.21 1 Mascot (2) 3 1038.08521 1.27 2953 2953 Image Peak List
High GMVSSGGPVsPGPVYPGGRPGAGGLMPGMPGTR CID S10(PhospR4) 33.81 1.00 1 Mascot (2) 3 1039.81946 -1.02 2290 2290 Image Peak List
High NVEGsNEDEEEEIEEDGEEISEVDHAK CID S5(PhospR4) 71.92 0.72 1 Mascot (2) 3 1047.41394 1.53 2225 2225 Image Peak List
High HDAIASGSPLPVQASGSPLSVQASKPADSsPK CID S30(PhospR4) 42.52 0.07 1 Mascot (2) 3 1056.18506 -0.36 1567 1567 Image Peak List
High DQQAVGYMANHEPLNSPLsPGYGLQsPR CID S19(PhospR4); S26(PhospR4) 46.38 0.07 1 Mascot (2) 3 1062.79968 2.89 2621 2621 Image Peak List
High RKGDGDDEEsEDESAENVVNVEDFLVQK CID S10(PhospR4) 49.62 1.00 1 Mascot (2) 3 1078.13159 -0.03 2612 2612 Image Peak List
High IEEEsENEEEEEGEEEDDDEEVKEKK CID S5(PhospR4) 69.27 1.00 1 Mascot (2) 3 1078.75342 2.43 421 421 Image Peak List
High HDAIASGsPLPVQAsGSPLSVQASKPADSSPK CID S8(PhospR4); S15(PhospR4) 23.81 0.21 1 Mascot (2) 3 1082.84448 3.33 1571 1571 Image Peak List
High ELALLNSNNLREEsPGPsGGGSVSPFNSSGK CID S14(PhospR4); S18(PhospR4) 37.99 0.32 1 Mascot (2) 3 1087.82312 2.63 2535 2535 Image Peak List
High HSSSIsPALsPQPLGSYGSYGQNIPMGSQR CID S6(PhospR4); S10(PhospR4) 40.38 0.40 1 Mascot (2) 3 1088.48230 2.88 2451 2451 Image Peak List
High NDPNYDsGEDAYDGLVDSPVSDPLNDYKK CID S7(PhospR4) 45.99 0.24 1 Mascot (2) 3 1094.79639 3.37 2591 2591 Image Peak List
High KFsEQNIGAPPSYEEAVSDSRsPVYSER CID S3(PhospR4); S22(PhospR4) 66.36 0.09 1 Mascot (2) 3 1097.14404 2.69 1814 1814 Image Peak List
High GMGTNLFDKPSHPNsPTVYDWLYSDDTR CID S15(PhospR4) 36.43 0.25 1 Mascot (2) 3 1098.48450 4.34 2843 2843 Image Peak List
High DHYDMYEPEANTMLQNSAPGsPFHPAGSR CID S21(PhospR4) 29.19 0.10 1 Mascot (2) 3 1100.45911 2.07 2124 2124 Image Peak List
High NGQIGIAHNPLWYEPYDPSDPDDVEGcNR CID C27(Methylthio) 44.00 1.00 1 Mascot (2) 3 1106.48132 0.91 2596 2596 Image Peak List
High TASNDESSsFDGHDEEDSVEHDESTADTAR CID S9(PhospR4) 47.49 0.19 1 Mascot (2) 3 1107.41553 1.93 773 773 Image Peak List
High sLTMPAAVSPGTPTTPTTPttPRKDNVWR CID S1(PhospR4); T20(PhospR4); T21(PhospR4) 21.15 1.00 1 Mascot (2) 3 1107.50134 2.11 2271 2271 Image Peak List
High KTDGsTTPAYAHGQHHSIFSPATGAVSDSSLK CID S5(PhospR4) 33.16 0.06 1 Mascot (2) 3 1112.51770 1.68 967 967 Image Peak List
High HDSSSSYKsDADDDVDFSETTSNPTWLEK CID S9(PhospR4) 23.34 0.04 1 Mascot (2) 3 1115.12183 2.21 1942 1942 Image Peak List
High YQSTYEGYGsPIREEPPPPYSYSEPQSR CID S10(PhospR4) 70.91 0.35 1 Mascot (2) 3 1115.48730 1.59 1784 1784 Image Peak List
High IWTRPSESETQTNSSEANPTTALLSTsFTK CID S27(PhospR4) 52.05 0.10 1 Mascot (2) 3 1122.19360 2.40 2358 2358 Image Peak List
High KEEATPAPAVVEtPVKEPETTTTAPVAEPPKP CID T13(PhospR4) 39.75 0.18 1 Mascot (2) 3 1131.23694 1.20 1326 1326 Image Peak List
High RAPGGSPTYSTGSsAHWDNVIPMGTLQPAPcK CID S14(PhospR4); C31(Methylthio) 22.25 1.00 1 Mascot (2) 3 1137.18018 2.78 2474 2474 Image Peak List
High STssSPPPPPPSSSLPQQEQEQDQQQLPLR CID S3(PhospR4); S4(PhospR4) 30.14 0.10 1 Mascot (2) 3 1144.17334 -4.26 2045 2045 Image Peak List
High GKPLEQAPLASSSsAPSLAAAASNATSTATEAPSVK CID S14(PhospR4) 78.01 0.15 1 Mascot (2) 3 1150.23657 3.10 2129 2129 Image Peak List
High STPAKNsDsEEMFDsDGEDEEEDKEVAVK CID S7(PhospR4); S9(PhospR4); S15(PhospR4) 41.41 0.39 1 Mascot (2) 3 1153.08752 0.10 1410 1410 Image Peak List
High sPEHALFTKPVYDQTEQLPPAPWETQEPR CID S1(PhospR4) 32.62 0.03 1 Mascot (2) 3 1157.88391 3.27 2470 2470 Image Peak List
High EIsHDsSDEEDGDGGKNTNEMLNYGLTIASK CID S3(PhospR4); S6(PhospR4) 28.70 0.03 1 Mascot (2) 3 1162.80457 1.78 2491 2491 Image Peak List
Medium STSssPPPPPPSSSLPQQEQEQDQQQLPLRR CID S4(PhospR4); S5(PhospR4) 17.53 1.00 1 Mascot (2) 3 1196.21582 3.26 1592 1592 Image Peak List
High EATADMsEEFsEGEKGDIISDISTHGESTKPR CID S7(PhospR4); S11(PhospR4) 50.08 0.40 1 Mascot (2) 3 1205.16968 0.50 2138 2138 Image Peak List
High VEEEEEEDEIVEsDVELEGDTVEPDNDPPQK CID S13(PhospR4) 46.55 0.51 1 Mascot (2) 3 1208.16956 2.43 2344 2344 Image Peak List
High SNASVGNGSVEDANTDASEVSKDDAGPAsEEEKSEA CID S29(PhospR4) 49.55 0.16 1 Mascot (2) 3 1211.83545 2.32 990 990 Image Peak List
High TGVNDDDDKNFKEEPEIQVLsDDDsDEEQVK CID S21(PhospR4); S25(PhospR4) 59.21 0.56 1 Mascot (2) 3 1238.17297 2.57 1833 1833 Image Peak List
High SSAVQEEQFGEHTEPcSSEIKDESHGKEESVEVK CID C16(Methylthio) 40.72 1.00 1 Mascot (2) 3 1274.56616 2.22 1086 1086 Image Peak List
High SKDNLYEQKPEEPVPVIPAAsPTNDTSAAGSSFASR CID S21(PhospR4) 44.84 0.02 1 Mascot (2) 3 1280.93982 2.17 1835 1835 Image Peak List
High SSVAEVTVPSSPYKHEDPTEPDsREEESPALGAIR CID S23(PhospR4) 51.81 0.08 1 Mascot (2) 3 1282.93250 3.17 1562 1562 Image Peak List
High IDLDKPEVEDDDDNDEDDsEDDDEAEGHDGEAGGR CID S19(PhospR4) 74.57 1.00 1 Mascot (2) 3 1304.80310 -4.44 1126 1126 Image Peak List
High FAAPSSLsEDDDDDDDDDPDYVEEEEEPLVSHRPR CID S8(PhospR4) 137.84 0.16 1 Mascot (2) 3 1362.54663 1.16 2078 2078 Image Peak List
High GMDVDEDDsDDDEEEDsEDEEEEETPKKPEPINK CID S9(PhospR4); S17(PhospR4) 104.14 0.24 1 Mascot (2) 3 1366.83130 1.30 955 955 Image Peak List

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Search Summary

Workflow created with Discoverer version: 1.2.0.208 (DBVersion:53)

================================================================================

Search name: F15_CS23_incl_032012_Excl2_MSA
Search description: -
Search date: 03/25/2013 10:20:42

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot

================================================================================

Search name: F15_CS23_incl_032012_Excl2_MSA
Search description: -
Search date: 03/25/2013 10:20:42

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot

------------------------------------------------------------------------------
Processing node 0: Spectrum Files
------------------------------------------------------------------------------

Input Data:
-----------------------------
File Name(s): F:\Raw files\032012\F15_CS23_incl_032012_Excl2.raw

------------------------------------------------------------------------------
Processing node 1: Spectrum Selector
------------------------------------------------------------------------------

1. General Settings:
-----------------------------
Precursor Selection: Use MS1 Precursor
Use New Precursor Reevaluation: True

2. Spectrum Properties Filter:
-----------------------------
Lower RT Limit: 0
Upper RT Limit: 0
Lowest Charge State: 0
Highest Charge State: 0
Min. Precursor Mass: 400 Da
Max. Precursor Mass: 10000 Da
Total Intensity Threshold: 0
Minimum Peak Count: 1

3. Scan Event Filters:
-----------------------------
MS Order: Is MS2
Activation Type: Is CID
Scan Type: Is Full

4. Peak Filters:
-----------------------------
S/N Threshold (FT-only): 3

5. Replacements for Unrecognized Properties:
-----------------------------
Unrecognized Charge Replacements: Automatic
Unrecognized Mass Analyzer Replacements: ITMS
Unrecognized MS Order Replacements: MS2
Unrecognized Activation Type Replacements: CID
Unrecognized Polarity Replacements: +

------------------------------------------------------------------------------
Processing node 2: Mascot
------------------------------------------------------------------------------

1. Input Data:
-----------------------------
Protein Database: TAIR10
Enzyme Name: Trypsin
Maximum Missed Cleavage Sites: 2
Instrument: ESI-TRAP
Taxonomy: All entries

1.1 Peptide Scoring Options:
-----------------------------
Peptide Cut Off Score: 10

1.2 Protein Scoring Options:
-----------------------------
Use MudPIT Scoring: True
Protein Relevance Threshold: 20
Protein Relevance Factor: 1

2. Decoy Database Search:
-----------------------------
Decoy Search: True

3. Tolerances:
-----------------------------
Precursor Mass Tolerance: 5 ppm
Fragment Mass Tolerance: 0.6 Da
Use Average Precursor Mass: False

4. Decoy Database Search:
-----------------------------
Significance High: 0.15
Significance Middle: 0.7505

4. Dynamic Modifications:
-----------------------------
1. Dynamic Modification: Phosphogood (ST)
2. Dynamic Modification: Phospho (Y)

5. Static Modifications:
-----------------------------
1. Static Modification: Methylthio (C)

================================================================================

Processing details:

03/25/2013 10:55 AM (2):Mascot: Search completed
03/25/2013 10:55 AM (2):Mascot: 430 protein(s) scored and inserted into result file in 9.14 s
03/25/2013 10:55 AM (2):Mascot: ScoreCalculator: 430 protein(s) scored
03/25/2013 10:55 AM (2):Mascot: Result finalization started
03/25/2013 10:55 AM (2):Mascot: Start calculating relaxed FDR
03/25/2013 10:55 AM (2):Mascot: Start calculating strict FDR
03/25/2013 10:55 AM (2):Mascot: Used mascot server http://mascot/mascot/ with Mascot version 2.2.7
03/25/2013 10:55 AM (2):Mascot: Sending 517 peptide hits (1124 peptides) to result file
03/25/2013 10:55 AM (2):Mascot: Sending 41 decoy peptide hits (66 peptides) to result file
03/25/2013 10:55 AM (2):Mascot: Reading decoy results
03/25/2013 10:55 AM (2):Mascot: Start translating results
03/25/2013 10:55 AM (2):Mascot: Start mapping modifications
03/25/2013 10:54 AM (2):Mascot: Received 521 proteins from Mascot server
03/25/2013 10:54 AM (2):Mascot: Start mapping 521 proteins
03/25/2013 10:54 AM (2):Mascot: Start parsing results
03/25/2013 10:54 AM (2):Mascot: Received Mascot result file (filename=../data/20130325/F007464.dat)
03/25/2013 10:54 AM (2):Mascot: Mascot Server completed
03/25/2013 10:52 AM (2):Mascot: Mascot result on server (filename=../data/20130325/F007464.dat)
03/25/2013 10:52 AM (2):Mascot: Start searching 3598 spectra
03/25/2013 10:52 AM (2):Mascot: Receiving spectra 3001 to 3599.
03/25/2013 10:52 AM (1):Spectrum Selector: Sending 598 spectra to Spectrum Distributor
03/25/2013 10:52 AM (1):Spectrum Selector: Sending 598 spectra to result file
03/25/2013 10:52 AM (2):Mascot: Receiving spectra 2001 to 3001.
03/25/2013 10:52 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:52 AM (1):Spectrum Selector: Sending 1000 spectra to result file
03/25/2013 10:52 AM (2):Mascot: Receiving spectra 1001 to 2001.
03/25/2013 10:52 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:51 AM (1):Spectrum Selector: Sending 1000 spectra to result file
03/25/2013 10:51 AM (2):Mascot: Use mascot server http://mascot/mascot/ with Mascot version 2.2.7
03/25/2013 10:51 AM (2):Mascot: Receiving spectra 1 to 1001.
03/25/2013 10:51 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:51 AM (1):Spectrum Selector: Sending 1000 spectra to result file


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