from cleavage_seq_selection import * #this command to call functions from cleavage_seq_selection.py # all filenames are subject to be modified by users inputS1 = [["input1.csv", "Fr.A2"], ["input2.csv", "Fr.B0"], ["input3.csv", "Fr.B1"], ["input4.csv.csv", "Fr.B3"]] #this command declares input files for selection step 1 in cleavage_seq_selection.py outputS1 = "qExHF01_05531_34_GluC_15uL.csv" #this command declares output filename for selection step 1 in cleavage_seq_selection.py CS_select1(inputS1, outputS1) #this command calls for execution of for selection step 1 in cleavage_seq_selection.py dbase = "database_name.csv" outputS2a = "Non-Prime.csv" outputS2b = "Prime.csv" outputS2c = "NPrime_potent.csv" outputS2d = "Prime_potent.csv" #these commands declare input and output files for selection step 2 in cleavage_seq_selection.py CS_select2(dbase, outputS1, outputS2a, outputS2b, outputS2c, outputS2d) #this command calls for execution of for selection step 1 in cleavage_seq_selection.py outputS3a = "NPrime_CS.csv" outputS3b = "Prime_CS.csv" #these commands declare input and output files for selection step 3 in cleavage_seq_selection.py CS_select3a(outputS2c, outputS3a) CS_select3b(outputS2d, outputS3b) #these commands call for execution of for selection step 3a and 3b in cleavage_seq_selection.py CS_select4(outputS3a, outputS3b, "Non-redundant_CSeq.csv") #this command calls for execution of for selection step 4 in cleavage_seq_selection.py