“Ultramixing”:
A Simple and Effective
Method To Obtain Controlled and Stable Dispersions of Graphene Oxide
in Cell Culture Media
Version 2 2019-02-12, 16:35
Version 1 2019-02-12, 15:03
Posted on 2019-02-12 - 16:35
The
last decade has seen an increase in the application of graphene
oxide (GO) in the biomedical field. GO has been successfully exploited
for its ability to deliver many kinds of drugs into target cells.
However, GO toxicity assessment is still controversial. Several studies
have demonstrated that GO protein coating is crucial to alleviate
the material’s toxicity. Besides, coronation leads to the formation
of big agglomerates, reducing the cellular uptake of the material
and thus its therapeutic efficiency. In this work, we propose a simple
and efficient method based on rapid (ultra-turrax, UT) mixing to control
protein corona formation. Using the UT protocol, we were able to reduce
GO agglomeration in the presence of proteins and obtain stable GO
dispersions in cell culture media. By labelling GO with luminescent
nanoparticles (quantum dots), we studied the GO internalization kinetic
and efficiency. Comparing the “classic” and UT protocols,
we found that the latter allows faster and more efficient internalization
both in macrophages and HeLa cells without affecting cell viability.
We believe that the use of UT protocol will be interesting and suitable
for the preparation of next-generation GO-based drug-delivery platforms.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Reina, Giacomo; Ruiz, Amalia; Murera, Diane; Nishina, Yuta; Bianco, Alberto (2019). “Ultramixing”:
A Simple and Effective
Method To Obtain Controlled and Stable Dispersions of Graphene Oxide
in Cell Culture Media. ACS Publications. Collection. https://doi.org/10.1021/acsami.8b18304
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (5)
GR
Giacomo Reina
AR
Amalia Ruiz
DM
Diane Murera
YN
Yuta Nishina
AB
Alberto Bianco