Thiol-Reactive Polymers for Titanium Interfaces: Fabrication
of Antimicrobial Coatings
Version 2 2019-06-04, 16:41
Version 1 2019-06-04, 16:38
Posted on 2019-06-04 - 16:41
Infection associated with surgical
implants is a major cause of their failure. Often in such cases, the
implant has to be removed and replaced, which causes undesirable patient
discomfort and complications. Bacterial adhesion and growth on implant
surface is the primary reason for such infections. Among the approaches
to prevent implant associated infections, the conjugation of antimicrobial
peptide (AMP) onto the surface of the implant is a very promising
approach. In this study, we describe a facile method for the surface
modification of titanium (Ti), a widely used material in dental and
orthopedic implants, to prevent bacterial adhesion and growth. Thin
polymeric films were synthesized on the Ti surface by using a copolymer
containing the maleimide group as a thiol-reactive handle to enable
the conjugation of AMPs. Robust attachment of the polymeric coating
on Ti surfaces was ensured through installation of catechol moieties
on the polymer as surface anchoring groups and the variation of the
amount of thiol-reactive maleimide group on titanium surfaces. As
a proof of concept, to demonstrate a viable application of such thiol
reactive surfaces, the antimicrobial peptide E6 (RRWRIVVIRVRRC)
was immobilized onto these well-characterized thin polymeric layers
through Michael addition. The antimicrobial activity of peptide-modified
surfaces was screened against both Gram-positive and Gram-negative
bacteria. The hydrophilic polymer coatings decreased the bacterial
adhesion, and the immobilized peptide killed >80% of the adhered
bacteria. The developed surface modification method has broad applicability
in terms of the choice of substrates and peptides in the design of
bioactive surfaces.
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Gevrek, Tugce Nihal; Yu, Kai; Kizhakkedathu, Jayachandran N.; Sanyal, Amitav (2019). Thiol-Reactive Polymers for Titanium Interfaces: Fabrication
of Antimicrobial Coatings. ACS Publications. Collection. https://doi.org/10.1021/acsapm.9b00117
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AUTHORS (4)
TG
Tugce Nihal Gevrek
KY
Kai Yu
JK
Jayachandran N. Kizhakkedathu
AS
Amitav Sanyal
KEYWORDS
Ti surfacesRobust attachmentAntimicrobial Coatings Infectionpeptide-modified surfacesbioactive surfacessurface modificationthiol-reactive maleimide groupantimicrobial peptide E 6AMPantimicrobial activitypolymer coatingsimplant surfaceGram-negative bacteriatitanium surfacescatechol moietiesMichael additionsurface modification methodBacterial adhesionThiol-Reactive Polymersantimicrobial peptideTitanium InterfacesRRWRImaleimide grouppatient discomfortTi surfacethiol reactive surfaces