Synthetic Biology Tools for the Fast-Growing Marine
Bacterium Vibrio natriegens
Posted on 2019-09-08 - 17:04
The fast-growing
nonmodel marine bacterium Vibrio natriegens has recently
garnered attention as a host for molecular biology
and biotechnology applications. In order to further its capabilities
as a synthetic biology chassis, we have characterized a wide range
of genetic parts and tools for use in V. natriegens. These parts include many commonly used resistance markers, promoters,
ribosomal binding sites, reporters, terminators, degradation tags,
origin of replication sequences, and plasmid backbones. We have characterized
the behavior of these parts in different combinations and have compared
their functionality in V. natriegens and Escherichia coli. Plasmid stability over time, plasmid copy
numbers, and production load on the cells were also evaluated. Additionally,
we tested constructs for chemical and optogenetic induction and characterized
basic engineered circuit behavior in V. natriegens. The results indicate that, while most parts and constructs work
similarly in the two organisms, some deviate significantly. Overall,
these results will serve as a primer for anyone interested in engineering V. natriegens and will aid in developing more robust synthetic
biology principles and approaches for this nonmodel chassis.
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