Straightforward Regeneration of Reduced Flavin Adenine
Dinucleotide Required for Enzymatic Tryptophan Halogenation
Version 2 2019-01-24, 00:46
Version 1 2019-01-17, 17:07
Posted on 2019-01-24 - 00:46
Flavin-dependent
halogenases are known to regioselectively introduce
halide substituents into aromatic moieties, for example, the indole
ring of tryptophan. The process requires halide salts and oxygen instead
of molecular halogen in the chemical halogenation. However, the reduced
cofactor flavin adenine dinucleotide (FADH2) has to be
regenerated using a flavin reductase. Consequently, coupled biocatalytic
steps are usually applied for cofactor regeneration. Nicotinamide
adenine dinucleotide (NADH) mimics can be employed stoichiometrically
to replace enzymatic cofactor regeneration in biocatalytic halogenation.
Chlorination of l-tryptophan is successfully performed using
such NADH mimics. The efficiency of this approach has been compared
to the previously established enzymatic regeneration system using
the two auxiliary enzymes flavin reductase (PrnF) and alcohol dehydrogenase
(ADH). The reaction rates of some of the tested mimics were found
to exceed that of the enzymatic system. Continuous enzymatic halogenation
reaction for reaction scale-up is also possible.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Ismail, Mohamed; Schroeder, Lea; Frese, Marcel; Kottke, Tilman; Hollmann, Frank; Paul, Caroline E.; et al. (2019). Straightforward Regeneration of Reduced Flavin Adenine
Dinucleotide Required for Enzymatic Tryptophan Halogenation. ACS Publications. Collection. https://doi.org/10.1021/acscatal.8b04500
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (7)
MI
Mohamed Ismail
LS
Lea Schroeder
MF
Marcel Frese
TK
Tilman Kottke
FH
Frank Hollmann
CP
Caroline E. Paul
NS
Norbert Sewald