New Insights into
Porcine Milk N‑Glycome
and the Potential Relation with Offspring Gut Microbiome
Version 2 2019-01-11, 18:33
Version 1 2019-01-08, 21:17
Posted on 2019-01-11 - 18:33
N-Glycans are an important source of milk oligosaccharides.
In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes. A potential for milk N-glycans to act as gut microbe regulators in suckling animals
has attracted considerable attention; however, sow milk N-glycans and their potential effects upon the piglet’s gut
microbes in vivo remain unknown. In the present study,
we profiled the milk N-glycans of Meishan and Yorkshire
sows during lactation using UPLC and a mass spectrometry-based glycome
method, and we explored the correlations between milk N-glycans and offspring gut microbiota. Twenty-two N-glycan structures were identified in sow milk, among which 36% (8
out of 22) were fucosylated, 41% (9 out of 22) were sialylated, and
14% (3 out of 22) were high mannosylated. An N-glycan
with a NeuGc structure (namely PNO20, GlcNAc4-Man3-Gal2-Fuc-Neu5Gc) was identified in sow milk for
the first time. No compositional differences between the two breeds
or between different lactation times were found in porcine milk N-linked oligosaccharides (PNOs); however, the abundances
of different structures within this class did vary. The relative abundances
of fucosylated PNO3 (GlcNAc4-Man3-Fuc) and sialylated
PNO18 (GlcNAc4-Man3-Gal2-NeuAc) increased
during lactation, and Meishan sows demonstrated a higher (P < 0.05) abundance of mannosylated PNO10 (GlcNAc2-Man6) and sialylated PNO17 (GlcNAc5-Man3-Gal-NeuAc) than Yorkshire sows. Apparent correlations
between milk N-glycans and offspring gut microbial
populations were found; for example, mannosylated PNO21 (GlcNAc2-Man9) was positively correlated with OTU706 (Lactobacillus amylovorus) and OTU1380 (Bacteroides
uniformis). Overall, our results indicate that the milk N-glycome of Meishan and Yorkshire sows differs in N-glycome characteristics and that this is correlated to
abundances of certain piglet gut microbes. These findings provide
a reference for future elucidation of the involvement of gut microbes
in milk N-glycan metabolism, which is important to
the health both of large domestic animals and humans.
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Mu, Chunlong; Cai, Zhipeng; Bian, Gaorui; Du, Yamin; Ma, Shouqing; Su, Yong; et al. (2019). New Insights into
Porcine Milk N‑Glycome
and the Potential Relation with Offspring Gut Microbiome. ACS Publications. Collection. https://doi.org/10.1021/acs.jproteome.8b00789
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AUTHORS (10)
CM
Chunlong Mu
ZC
Zhipeng Cai
GB
Gaorui Bian
YD
Yamin Du
SM
Shouqing Ma
YS
Yong Su
LL
Li Liu
JV
Josef Voglmeir
RH
Ruihua Huang
WZ
Weiyun Zhu