Naked-Eye Detection of Grapevine Red-Blotch Viral
Infection Using a Plasmonic CRISPR Cas12a Assay
Posted on 2019-09-05 - 17:40
Herein,
we described a novel plasmonic CRISPR Cas12a assay for
the visual, colorimetric detection of grapevine viral infections.
Our assay generates rapid and specific colorimetric signals for nucleic
acid amplicons by combining the unique target-induced incriminate
single-stranded DNase activity of Cas12a with plasmon coupling of
DNA functionalized gold nanoparticles. The practical applicability
of our plasmonic assay was successfully demonstrated through the detection
of emerging red-blotch viral infections in grapevine samples collected
from commercial vineyards.
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Li, Yongya; Mansour, Hayam; Wang, Tony; Poojari, Sudarsana; Li, Feng (2019). Naked-Eye Detection of Grapevine Red-Blotch Viral
Infection Using a Plasmonic CRISPR Cas12a Assay. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.9b03545
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AUTHORS (5)
- YLYongya LiHMHayam MansourTWTony WangSPSudarsana PoojariFLFeng Li
KEYWORDS
Grapevine Red-Blotch Viral Infectionnovel plasmonic CRISPR Cas 12a assaygrapevine samplesNaked-Eye Detectionacid ampliconscolorimetric signalstarget-induced incriminate single-stranded DNase activityCas 12ainfectioncolorimetric detectionDNA functionalized gold nanoparticlesPlasmonic CRISPR Cas 12a Assay Hereinplasmonic assay