Multiplexed Detection of Tumor Markers with Multicolor
Polymer Dot-Based Immunochromatography Test Strip
Version 2 2018-01-16, 20:14
Version 1 2018-01-16, 20:10
Posted on 2018-01-16 - 20:14
There
have been ongoing efforts to develop more sensitive and fast
quantitative screening of cancer markers by use of fluorometric immunochromatographic
test strips (ICTS) since the remarkable advances in fluorescent nanomaterials.
Semiconducting polymer dots (Pdots) have recently emerged as a new
type of biocompatible fluorescent probe with extraordinary brightness
which is suitable for biological and clinical use. Here, we developed
Pdot-based ICTS for quantitative rapid screening of prostate-specific
antigen (PSA), α-fetoprotein (AFP), and carcinoembryonic antigen
(CEA) in 10 min. Through use of the ultrahigh fluorescence brightness
of Pdots, this immunosensor enabled much better detection sensitivity
(2.05, 3.30, and 4.92 pg/mL for PSA, AFP, and CEA, respectively),
in which the detection limit is at least 2 orders of magnitude lower
than that of conventional fluorometric ICTS. Furthermore, we performed
proof-of-concept experiments for simultaneous determination of multiple
tumor markers in a single test strip. These results demonstrated that
this Pdot-based ICTS platform is a promising candidate for developing
new generations of point-of-care diagnostics. To the best of our knowledge,
this is the first example of Pdot-based ICTS with multiplexing capability.
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Fang, Chia-Chia; Chou, Chia-Cheng; Yang, Yong-Quan; Wei-Kai, Tsai; Wang, Yeng-Tseng; Chan, Yang-Hsiang (2018). Multiplexed Detection of Tumor Markers with Multicolor
Polymer Dot-Based Immunochromatography Test Strip. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.7b04411
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AUTHORS (6)
CF
Chia-Chia Fang
CC
Chia-Cheng Chou
YY
Yong-Quan Yang
TW
Tsai Wei-Kai
YW
Yeng-Tseng Wang
YC
Yang-Hsiang Chan