Molecular Design of Polyampholytes for Vitrification-Induced
Preservation of Three-Dimensional Cell Constructs without Using Liquid
Nitrogen
Version 2 2020-07-30, 16:42
Version 1 2020-07-27, 18:42
Posted on 2020-07-30 - 16:42
Current
slow-freezing methods are too inefficient for cryopreservation
of three-dimensional (3D) tissue constructs. Additionally, conventional
vitrification methods use liquid nitrogen, which is inconvenient and
increases the chance of cross-contamination. Herein, we have developed
polyampholytes with various degrees of hydrophobicity and showed that
they could successfully vitrify cell constructs including spheroids
and cell monolayers without using liquid nitrogen. The polyampholytes
prevented ice crystallization during both cooling and warming, demonstrating
their potential to prevent freezing-induced damage. Monolayers and
spheroids vitrified in the presence of polyampholytes yielded high
viabilities post-thawing with monolayers vitrified with PLL-DMGA exhibiting
more than 90% viability. Moreover, spheroids vitrified in the presence
of polyampholytes retained their fusibilities, thus revealing the
propensity of these polyampholytes to stabilize 3D cell constructs.
This study is expected to open new avenues for the development of
off-the-shelf tissue engineering constructs that can be prepared and
preserved until needed.
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Matsumura, Kazuaki; Hatakeyama, Sho; Naka, Toshiaki; Ueda, Hiroshi; Rajan, Robin; Tanaka, Daisuke; et al. (2020). Molecular Design of Polyampholytes for Vitrification-Induced
Preservation of Three-Dimensional Cell Constructs without Using Liquid
Nitrogen. ACS Publications. Collection. https://doi.org/10.1021/acs.biomac.0c00293
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AUTHORS (7)
KM
Kazuaki Matsumura
SH
Sho Hatakeyama
TN
Toshiaki Naka
HU
Hiroshi Ueda
RR
Robin Rajan
DT
Daisuke Tanaka
SH
Suong-Hyu Hyon