Mitochondria Penetrating Peptide-Conjugated TAMRA
for Live-Cell Long-Term Tracking
Posted on 2019-08-23 - 12:33
Mitochondria
are essential targets for treatment of diseases with
mitochondrial disorders such as diabetes, cancer, and cardiovascular
and neurodegenerative diseases. Mitochondria penetrating peptides
(MPPs) are composed of cationic and hydrophobic amino acids that can
target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based
fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited
advantageous properties for long-term mitochondria tracking as demonstrated
by fluorescence microscopy. Cell viability assays and oxygen consumption
rates indicate low cytotoxicity and high biocompatibility of the new
contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is
a promising candidate for continuous mitochondrial tracking for up to 3 days.
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Zhao, Tinghan; Liu, Xinglei; Singh, Sweety; Liu, Xiangshan; Zhang, Yuanwei; Sawada, Junko; et al. (2019). Mitochondria Penetrating Peptide-Conjugated TAMRA
for Live-Cell Long-Term Tracking. ACS Publications. Collection. https://doi.org/10.1021/acs.bioconjchem.9b00465
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AUTHORS (8)
TZ
Tinghan Zhao
XL
Xinglei Liu
SS
Sweety Singh
XL
Xiangshan Liu
YZ
Yuanwei Zhang
JS
Junko Sawada
MK
Masanobu Komatsu
KB
Kevin D. Belfield
KEYWORDS
neurodegenerative diseasesnovel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH 2Live-Cell Long-Term Tracking MitochondriaTAMRA-rFrFrFoxygen consumption ratescontrast agentmitochondrial disordersColocalization studiescell viability assays3 daysMitochondria Penetrating Peptide-Conjugated TAMRAmitochondrial membranefluorescence microscopyMPP