Ipomoeassin
F Binds Sec61α to Inhibit Protein
Translocation
Version 2 2019-07-11, 23:43
Version 1 2019-05-15, 19:15
Posted on 2019-07-11 - 23:43
Ipomoeassin F is a potent natural
cytotoxin that inhibits growth
of many tumor cell lines with single-digit nanomolar potency. However,
its biological and pharmacological properties have remained largely
unexplored. Building upon our earlier achievements in total synthesis
and medicinal chemistry, we used chemical proteomics to identify Sec61α
(protein transport protein Sec61 subunit alpha isoform 1), the pore-forming
subunit of the Sec61 protein translocon, as a direct binding partner
of ipomoeassin F in living cells. The interaction is specific and
strong enough to survive lysis conditions, enabling a biotin analogue
of ipomoeassin F to pull down Sec61α from live cells, yet it
is also reversible, as judged by several experiments including fluorescent
streptavidin staining, delayed competition in affinity pulldown, and
inhibition of TNF biogenesis after washout. Sec61α forms the
central subunit of the ER protein translocation complex, and the binding
of ipomoeassin F results in a substantial, yet selective, inhibition
of protein translocation in vitro and a broad ranging
inhibition of protein secretion in live cells. Lastly, the unique
resistance profile demonstrated by specific amino acid single-point
mutations in Sec61α provides compelling evidence that Sec61α
is the primary molecular target of ipomoeassin F and strongly suggests
that the binding of this natural product to Sec61α is distinctive.
Therefore, ipomoeassin F represents the first plant-derived, carbohydrate-based
member of a novel structural class that offers new opportunities to
explore Sec61α function and to further investigate its potential
as a therapeutic target for drug discovery.
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Zong, Guanghui; Hu, Zhijian; O’Keefe, Sarah; Tranter, Dale; Iannotti, Michael J.; Baron, Ludivine; et al. (2019). Ipomoeassin
F Binds Sec61α to Inhibit Protein
Translocation. ACS Publications. Collection. https://doi.org/10.1021/jacs.8b13506
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AUTHORS (23)
GZ
Guanghui Zong
ZH
Zhijian Hu
SO
Sarah O’Keefe
DT
Dale Tranter
MI
Michael J. Iannotti
LB
Ludivine Baron
BH
Belinda Hall
KC
Katherine Corfield
AP
Anja O. Paatero
MH
Mark J. Henderson
PR
Peristera Roboti
JZ
Jianhong Zhou
XS
Xianwei Sun
MG
Mugunthan Govindarajan
JR
Jason M. Rohde
NB
Nicolas Blanchard
RS
Rachel Simmonds
JI
James Inglese
YD
Yuchun Du
CD
Caroline Demangel