Intrinsic
Color Sensing System Allows for Real-Time
Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived
Cardiomyocytes
Posted on 2020-07-08 - 20:31
Stem-cell
based in vitro differentiation for disease
modeling offers great value to explore the molecular and functional
underpinnings driving many types of cardiomyopathy and congenital
heart diseases. Nevertheless, one major caveat in the application
of in vitro differentiation of human induced pluripotent
stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) involves the
immature phenotype of the CMs. Most of the existing methods need complex
apparatus and require laborious procedures in order to monitor the
cardiac differentiation/maturation process and often result in cell
death. Here we developed an intrinsic color sensing system utilizing
a microgroove structural color methacrylated gelatin film, which allows
us to monitor the cardiac differentiation process of hiPSC-derived
cardiac progenitor cells in real time. Subsequently this system can
be employed as an assay system to live monitor induced functional
changes on hiPSC-CMs stemming from drug treatment, the effects of
which are simply revealed through color diversity. Our research shows
that early intervention of cardiac differentiation through simple
physical cues can enhance cardiac differentiation and maturation to
some extent. Our system also simplifies the previous complex experimental
processes for evaluating the physiological effects of successful differentiation
and drug treatment and lays a solid foundation for future transformational
applications.
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Gong, Yiqi; Chen, Zhuoyue; Yang, Li; Ai, Xuefeng; Yan, Bingqian; Wang, Huijing; et al. (2020). Intrinsic
Color Sensing System Allows for Real-Time
Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived
Cardiomyocytes. ACS Publications. Collection. https://doi.org/10.1021/acsnano.0c01745
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AUTHORS (12)
YG
Yiqi Gong
ZC
Zhuoyue Chen
LY
Li Yang
XA
Xuefeng Ai
BY
Bingqian Yan
HW
Huijing Wang
LQ
Liya Qiu
YT
Yao Tan
NW
Nevin Witman
WW
Wei Wang
YZ
Yuanjin Zhao
WF
Wei Fu