Insights into the Inhibition Mechanism of Human Pancreatic
α‑Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol
B Isolated from Ocimum tenuiflorum
Posted on 14.01.2021 - 01:13
Use of human pancreatic α-amylase (HPA) inhibitors is one of the effective antidiabetic strategies to lower postprandial hyperglycemia via reduction in the dietary starch hydrolysis rate. Many natural products from plants are being studied for their HPA inhibitory activity. The present study describes isolation of dehydrodieugenol B (DDEB) from Ocimum tenuiflorum leaves using sequential solvent extraction, structure determination by one-dimensional (1D) and two-dimensional (2D) NMR analyses, and characterization as an HPA inhibitor using kinetics, binding thermodynamics, and molecular docking. DDEB uncompetitively inhibited HPA with an IC50 value of 29.6 μM for starch and apparent Ki′ of 2.49 and Ki of 47.6 μM for starch and maltopentaose as substrates, respectively. The circular dichroism (CD) study indicated structural changes in HPA on inhibitor binding. Isothermal titration calorimetry (ITC) revealed thermodynamically favorable binding (ΔG of −7.79 kcal mol–1) with a dissociation constant (Kd) of 1.97 μM and calculated association constant (Ka) of 0.507 μM. Molecular docking showed stable HPA–inhibitor binding involving H-bonds and Pi-alkyl, alkyl–alkyl, and van der Waals (vDW) interactions. The computational docking results support the noncompetitive nature of DDEB binding. The present study could be helpful for exploration of the molecule as a potential antidiabetic drug candidate to control postprandial hyperglycemia.
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Dandekar, Prasad D.; Kotmale, Amol S.; Chavan, Shrawan R.; Kadlag, Pranita P.; Sawant, Sangeeta V.; Dhavale, Dilip D.; et al. (1753): Insights into the Inhibition Mechanism of Human Pancreatic α‑Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol B Isolated from Ocimum tenuiflorum. ACS Publications. Collection. https://doi.org/10.1021/acsomega.0c00617
Prasad D. Dandekar
Amol S. Kotmale
Shrawan R. Chavan
Pranita P. Kadlag
Sangeeta V. Sawant
Dilip D. Dhavale