Impeded Molecular Reorganization by Polyethylene Glycol
Conjugation Revealed by X‑ray Reflectivity and Diffraction
Measurements
Posted on 2020-06-17 - 16:15
Polyethylene glycol (PEG) coatings
have been widely applied in
pharmaceutical and biomedical systems to prevent nonspecific protein
absorption, increase vesicle blood circulation time, and sustain drug
release. This study systematically investigated the planar interfacial
organization of phospholipid monolayers containing various amounts
of PEG conjugations before and after enzyme-catalyzed degradation
of the lipids using X-ray reflectivity and grazing incidence X-ray
diffraction techniques. Results showed that attaching PEG to the headgroup
of the lipids up to 15 mol % had limited effects on molecular packing
of the lipid monolayers in the condensed phase at the gas–liquid
interface and negligible effects on the enzyme adsorption to the interface.
After enzyme-catalyzed degradation, equimolar fatty acids and lyso
PC were generated. The fatty acids together with the subphase Ca2+ self-assembled into highly organized multilayer domains
at the interface. The X-ray measurements unambiguously revealed that
the densely packed PEG markedly hindered microphase separation and
formation of the palmitic acid–Ca2+ complexes.
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Zhang, Pin; Pham, Tiep; Liu, Chang; Leon Plata, Paola; Kalkowski, Joseph; Cheng, Gang; et al. (2020). Impeded Molecular Reorganization by Polyethylene Glycol
Conjugation Revealed by X‑ray Reflectivity and Diffraction
Measurements. ACS Publications. Collection. https://doi.org/10.1021/acs.langmuir.0c01202