Fluorescent Mn:ZnCdS@ZnS and CdTe Quantum Dots Probes
on SiO2 Microspheres for Versatile Detection of Carcinoembryonic
Antigen and Monitoring T4 Polynucleotide Kinase Activity
Version 2 2019-06-28, 12:34
Version 1 2019-06-28, 12:33
Posted on 2019-06-28 - 12:34
Versatile fluorescent quantum dots
(QDs) probes on SiO2 microspheres were prepared and used
for detection of carcinoembryonic
antigen (CEA) and monitoring T4 polynucleotide kinase (PNK) activity
by integrating with amplification strategy. The highly fluorescent
Mn:ZnCdS@ZnS and CdTe QDs showed different emission peaks. The amino-modified
SiO2 microspheres with good morphology and no fluorescence
interference were designed to link DNA and fabricate multiple branched
QDs probes. First, CEA-induced target recycle amplification technique
was combined with the Mn:ZnCdS@ZnS QDs labeled P2 signal probe for
sensitive detection of CEA. Then, the formed DNA S1–DNA3 double
strands further polymerized and proceeded exonuclease-cleavage reaction
in the presence of T4 PNK, the released DNA fragments were used to
trigger assembly of multibranched CdTe QDs-DNA signal probe (P1) by
hybridization chain reaction (HCR), and achieved a greatly amplified
fluorescence detection of PNK. The detection limits for CEA and PNK
were 0.1 pg/mL and 0.001 U/mL, which was comparable to the reported
methods. It is for the first time that the multiple branched QDs probes
was coupled with HCR and cycling amplification strategy for versatile
fluorescence detection of CEA and PNK activity. It shows great potential
for early clinical diagnosis of cancer and the nucleotide kinase-target
drug discovery.
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Jie, Guifen; Li, Chunli; Zhao, Yu; Kuang, Qian; Niu, Shuyan (2019). Fluorescent Mn:ZnCdS@ZnS and CdTe Quantum Dots Probes
on SiO2 Microspheres for Versatile Detection of Carcinoembryonic
Antigen and Monitoring T4 Polynucleotide Kinase Activity. ACS Publications. Collection. https://doi.org/10.1021/acsanm.9b01003
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AUTHORS (5)
GJ
Guifen Jie
CL
Chunli Li
YZ
Yu Zhao
QK
Qian Kuang
SN
Shuyan Niu
KEYWORDS
amino-modified SiO 2 microspherescycling amplification strategynucleotide kinase-target drug discoverySiO 2 microspheresCEAHCRMnQDs probesmultibranched CdTe QDs-DNA signal probemonitoring T 4 polynucleotide kinaseP 2 signal probeSiO 2 MicrospheresCdTe Quantum Dots Probeshybridization chain reactionMonitoring T 4 Polynucleotide Kinase Activity VersatileT 4 PNKfluorescence detection