Fluorescent
Membrane Tension Probes for Super-Resolution
Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent
Ketone Chemistry
Posted on 2020-07-01 - 16:43
We report the design, synthesis,
and evaluation of fluorescent
flipper probes for single-molecule super-resolution imaging of membrane
tension in living cells. Reversible switching from bright-state ketones
to dark-state hydrates, hemiacetals, and hemithioacetals is demonstrated
for twisted and planarized mechanophores in solution and membranes.
Broadband femtosecond fluorescence up-conversion spectroscopy evinces
ultrafast chalcogen-bonding cascade switching in the excited state
in solution. According to fluorescence lifetime imaging microscopy,
the new flippers image membrane tension in live cells with record
red shifts and photostability. Single-molecule localization microscopy
with the new tension probes resolves membranes well below the diffraction
limit.
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García-Calvo, José; Maillard, Jimmy; Fureraj, Ina; Strakova, Karolina; Colom, Adai; Mercier, Vincent; et al. (2020). Fluorescent
Membrane Tension Probes for Super-Resolution
Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent
Ketone Chemistry. ACS Publications. Collection. https://doi.org/10.1021/jacs.0c04942
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AUTHORS (11)
JG
José García-Calvo
JM
Jimmy Maillard
IF
Ina Fureraj
KS
Karolina Strakova
AC
Adai Colom
VM
Vincent Mercier
AR
Aurelien Roux
EV
Eric Vauthey
NS
Naomi Sakai
AF
Alexandre Fürstenberg
SM
Stefan Matile
KEYWORDS
Single-molecule localization microscopyflippers image membrane tensiontension probesflipper probesdiffraction limitfluorescence lifetime imaging micro...single-molecule super-resolution im...Super-Resolution MicroscopyBroadband femtosecond fluorescence ...bright-state ketonesDynamic-Covalent Ketone Chemistrydark-state hydratesmembrane tensionplanarized mechanophoresMechanosensitive Cascade SwitchingFluorescent Membrane Tension Probes