Exploration of the Selectivity and Retention Behavior
of Alternative Polyacrylamides in Temperature Responsive Liquid Chromatography
Posted on 2020-07-10 - 16:03
Temperature responsive
liquid chromatography (TRLC) allows for
separation of organic solutes in purely aqueous mobile phases whereby
retention is controlled through temperature. The vast majority of
the work has thus far been performed on poly[N-isopropylacrylamide]
(PNIPAAm)-based columns, while the performance of other temperature
responsive polymers has rarely been compared under identical conditions.
Therefore, in this work, two novel TRLC phases based on poly[N-n-propylacrylamide] (PNNPAAm) and poly[N,N-diethylacrylamide] (PDEAAm) are reported and compared
to the state of the art PNIPAAm based column. Optimal comparison is
thereby obtained by the use of controlled radical polymerizations,
identical molecular weights, and by maximizing carbon loads on the
silica supporting material. Analysis of identical test mixtures of
homologue series and pharmaceutical samples revealed that PNNPAAm
performs in a similar way as PNIPAAm while offering enhanced retention
and a shift of the useable temperature range toward lower temperatures.
PDEAAm offers a range of novel possibilities as it depicts a different
selectivity, allowing for enhanced resolution in TRLC in, for example,
coupled column systems. Reduced plate heights of 3 could be obtained
on the homemade columns, offering the promise for reasonable column
efficiencies in TRLC despite the use of bulky polymers as stationary
phases in HPLC.
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Baert, Mathijs; Wicht, Kristina; Hou, Zhanyao; Szucs, Roman; Prez, Filip Du; Lynen, Frederic (2020). Exploration of the Selectivity and Retention Behavior
of Alternative Polyacrylamides in Temperature Responsive Liquid Chromatography. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.0c01321
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AUTHORS (6)
MB
Mathijs Baert
KW
Kristina Wicht
ZH
Zhanyao Hou
RS
Roman Szucs
FP
Filip Du Prez
FL
Frederic Lynen
KEYWORDS
retentionPDEAAmHPLChomologue seriesTemperature Responsive Liquid Chrom...Optimal comparisoncolumn efficienciesnovel TRLC phasestest mixturestemperature rangepolycarbon loadsPNNPAAmofferingcolumn systemsReduced plate heightspolymerart PNIPAAmnovel possibilitiesRetention BehaviorAlternative Polyacrylamides