Evaluation of His6‑Metal Assemblies
as a Drug Delivery Vehicle in the Treatment of Anterior Segment Disease
Using a Corneal Inflammation Model
Posted on 2020-06-11 - 16:03
Keratitis
is a common ophthalmological disease and also a common
cause of blindness (second only to cataracts). This disease is routinely
treated by topical administration of dexamethasone sodium phosphate
(Dexp). However, due to the presence of anatomical and physiological
barriers, frequent administration is needed, often resulting in poor
patient compliance and diverse side effects. In this work, Dexp was in situ encapsulated into a His6-metal assembly
(HmA) to generate Dexp@HmA, which was utilized in the ocular delivery
of Dexp. The physicochemical properties of HmA and Dexp@HmA particles
were characterized in detail using various techniques such as dynamic
light scattering (DLS), scanning electron microscopy (SEM), and UV–vis
spectroscopy. Compared to commercial Eudragi and reported PLGA nanoparticles,
HmA showed higher encapsulation efficiency (EE%) and higher loading
capacity (LC wt %) of Dexp. Dexp@HmA displayed pH-dependent release;
after 33 days at pH 5.8, 6.5, and 7.2, 100%, 65%, and 42% of Dexp,
respectively, had been released. In addition, HmA and Dexp@HmA showed
low cytotoxicity to macrophages and to all common ocular cell types
tested. The effect of Dexp@HmA on corneal inflammation was evaluated
using in vitro and in vivo models.
Our results demonstrate that Dexp@HmA is much superior to free Dexp
in both in vitro and in vivo models.
These positive results suggest that HmA may represent a promising
candidate nanocarrier for the treatment of various diseases of the
anterior segment of the eye.
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Wang, Liwen; Luo, Shan; Xu, Hongyan; Wu, Xiaoxiao; Hao, Pengyan; Zhang, Yagang; et al. (2020). Evaluation of His6‑Metal Assemblies
as a Drug Delivery Vehicle in the Treatment of Anterior Segment Disease
Using a Corneal Inflammation Model. ACS Publications. Collection. https://doi.org/10.1021/acsbiomaterials.0c00218