Evaluation of Feruloylated and p‑Coumaroylated
Arabinosyl Units in Grass Arabinoxylans by Acidolysis in Dioxane/Methanol
Version 2 2018-05-18, 19:18
Version 1 2018-05-18, 17:52
Posted on 2018-05-18 - 19:18
The
arabinosyl side chains of grass arabinoxylans are partially
acylated by p-coumarate (pCA) and
ferulate (FA). These aromatic side chains can cross-couple wall polymers
resulting in modulation of cell wall physical properties. The determination
of p-coumaroylated and feruloylated arabinose units
has been the target of analytical efforts with trifluoroacetic acid
hydrolysis the standard method to release feruloylated and p-coumaroylated arabinose units from arabinoxylans. Herein,
we report on a more robust method to measure these acylated units.
Acidolysis of extractive-free grass samples in a dioxane/methanol/aqueous
2 M HCl mixture provided the methyl 5-O-p-coumaroyl- and 5-O-feruloyl-l-arabinofuranoside
anomers (pCA-MeAra and FA-MeAra). These conjugates
were readily analyzed by liquid chromatography combined with both
UV and MS detection. The method revealed the variability of the relative
acylation of arabinose units by pCA or FA in grass
cell walls. This methodology will permit delineation of hydroxycinnamate
acylation patterns in arabinoxylans.
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Lapierre, Catherine; Voxeur, Aline; Karlen, Steven D.; Helm, Richard F.; Ralph, John (2018). Evaluation of Feruloylated and p‑Coumaroylated
Arabinosyl Units in Grass Arabinoxylans by Acidolysis in Dioxane/Methanol. ACS Publications. Collection. https://doi.org/10.1021/acs.jafc.8b01618
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AUTHORS (5)
CL
Catherine Lapierre
AV
Aline Voxeur
SK
Steven D. Karlen
RH
Richard F. Helm
JR
John Ralph