Engineering Reversible Cell–Cell Interactions
with Lipid Anchored Prosthetic Receptors
Version 2 2018-03-23, 20:07
Version 1 2018-03-23, 20:03
Posted on 2018-03-23 - 20:07
Membrane-engineered
cells displaying antigen-targeting ligands
are useful as both scientific tools and clinical therapeutics. While
genetically encoded artificial receptors have proven efficacious,
their scope remains limited, as this approach is not amenable to all
cell types and the modification is often permanent. Our group has
developed a nongenetic method to rapidly, stably, and reversibly modify
any cell membrane with a chemically self-assembled nanoring (CSAN)
that can function as a prosthetic receptor. Bifunctional CSANs displaying
epithelial cell adhesion molecule (EpCAM)-targeted fibronectin domains
were installed on the cell membrane through hydrophobic insertion
and remained stably bound for ≥72 h in vitro. These CSAN-labeled
cells were capable of recognizing EpCAM-expressing target cells, forming
intercellular interactions that were subsequently reversed by disassembling
the nanoring with the FDA-approved antibiotic, trimethoprim. This
study demonstrates the use of this system to engineer cell surfaces
with prosthetic receptors capable of directing specific and reversible
cell–cell interactions.
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Csizmar, Clifford
M.; Petersburg, Jacob R.; Hendricks, Alex; Stern, Lawrence A.; J. Hackel, Benjamin; Wagner, Carston R. (2018). Engineering Reversible Cell–Cell Interactions
with Lipid Anchored Prosthetic Receptors. ACS Publications. Collection. https://doi.org/10.1021/acs.bioconjchem.8b00058
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AUTHORS (6)
CC
Clifford
M. Csizmar
JP
Jacob R. Petersburg
AH
Alex Hendricks
LS
Lawrence A. Stern
BJ
Benjamin J. Hackel
CW
Carston R. Wagner