Elevation of Condensed Tannins in the Leaves of Ta-MYB14‑1
White Clover (Trifolium repens L.) Outcrossed with
High Anthocyanin Lines
Posted on 2019-07-23 - 00:29
Condensed tannins (CT) are highly
desirable in forage as they sequester
dietary protein and reduce bloat and methane emissions in ruminants.
However, the widely used forage legume white clover (Trifolium
repens) only produces CTs in flowers and trichomes and at
levels too low to achieve therapeutic effects. Genetic transformation
with transcription factor Ta-MYB14-1 from Trifolium arvense was effective in inducing CTs to 0.6%
of leaf dry matter. CT synthesis has been elevated further by crossing
the primary white clover transgenic line with wild type genotypes
producing the related phenylpropanoids, anthocyanins. CT levels in
leaves were highest under the anthocyanin leaf marks associated with
the “red midrib” trait; however, there was no evidence
for CT accumulation in leaf sections with the “red V”
anthocyanin marking. Ta-MYB14-1 was stably inherited
in two generations of crosses, and T2 progeny produced up to 3.6-fold
higher CTs than the T0 parent. The profile of small CT oligomers such
as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny
and consisted predominantly of prodelphinidins (PD), with lesser amounts
of procyanidins (PC) and mixed PC:PD oligomers.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Roldan, Marissa B.; Cousins, Greig; Fraser, Karl; Hancock, Kerry R.; Collette, Vern; Demmer, Jerome; et al. (2019). Elevation of Condensed Tannins in the Leaves of Ta-MYB14‑1
White Clover (Trifolium repens L.) Outcrossed with
High Anthocyanin Lines. ACS Publications. Collection. https://doi.org/10.1021/acs.jafc.9b01185
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (10)
MR
Marissa B. Roldan
GC
Greig Cousins
KF
Karl Fraser
KH
Kerry R. Hancock
VC
Vern Collette
JD
Jerome Demmer
DW
Derek R. Woodfield
JC
John R. Caradus
CJ
Chris Jones
CR
Christine R. Voisey
KEYWORDS
methane emissionstranscription factor Ta-MYB 14-1BC 2 progenyCT synthesisanthocyanin leaf marksPCPDCT accumulationCT oligomersforage legumeGenetic transformationTrifolium arvenseHigh Anthocyanin Lines Condensed tanninsT 0 parentT 2 progenyleaf sectionstype genotypesCondensed TanninsTa-MYB 14-1Trifolium repensCT levels