Electrochemiluminescence Immunoassay Platform with
Immunoglobulin G‑Encapsulated Gold Nanoclusters as a “Two-In-One”
Posted on 15.09.2021 - 09:29
Biomolecule-functionalized Au nanoclusters (AuNCs) have drawn great interest in the electrochemiluminescence (ECL) field due to their unique optical/electrical properties, biocompatibility, and versatile bioapplication potentials. Herein, we proposed a two-in-one ECL probe of immunoglobulin G-encapsulated AuNCs (IgG-AuNCs) for the development of a high-performance ECL immunoassay (ECLIA) platform. The IgG-AuNCs were not only used as an ECL probe due to their excellent anodic ECL performance with triethylamine (TEA) as a coreactant but also used as the biorecognition element because of their well-retained bioactivity of the IgG. As a proof of concept, a new type of competitive immunosensing platform has been applied to detect IgG representing several merits of facile preparation, rapid detection, sample saving, and good analytical performance. The sensing platform exhibited a linear range of 0.5–50,000 ng/mL with a limit of detection of 0.06 ng/mL for IgG detection with high selectivity. In addition, this convenient ECLIA platform also performed well in real serum sample detection. Notably, our work not only proved the “two-in-one” immuno-AuNC probe-based ECLIA strategy but also developed a rational framework for study of ECL bioassay platforms based on multifunctional AuNCs and other related nanomaterials.
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Hong, Guolin; Su, Canping; Huang, Zhongnan; Zhuang, Quanquan; Wei, Chaoguo; Deng, Haohua; et al. (1753): Electrochemiluminescence Immunoassay Platform with Immunoglobulin G‑Encapsulated Gold Nanoclusters as a “Two-In-One” Probe. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.1c02850
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versatile bioapplication potentialsone ” immunogood analytical performancefunctionalized au nanoclustersdrawn great interest5 – 50sensing platform exhibitedperformance ecl immunoassaycompetitive immunosensing platformone ecl probebased eclia strategyecl probe dueelectrochemiluminescence immunoassay platformaunc probefield dueunique opticalsample savingretained bioactivityrelated nanomaterialsrational frameworknew typelinear rangeimmunoglobulin gfacile preparationelectrical propertiesbiorecognition elementalso developed06 ng000 ng